基于基因表达谱的肝细胞癌过表达基因筛选

Screening of the overexpressed genes in hepatocellular carcinoma based on the gene expression profiles

目的利用基因表达谱芯片技术筛选肝细胞癌(HCC)中表达上调最为明显的基因。方法利用Affymetrix U133 Plus2.0表达芯片检测93例HCC及配对癌旁肝组织的全基因表达谱。HCC与配对癌旁肝组织中基因表达的比较采用配对t检验,筛选表达上调最为明显的10个基因。各基因表达与临床病理参数的关系采用χ~2检验。结果与癌旁肝组织相比,HCC中表达上调倍数最多的10个基因分别为:CDKN3、CCL20、PTTG1、MELK、PRMT1、TOP2A、TPX2、SMYD3、TMEM106C和UCK2,上调25.2~44.6倍(均P≤2.0×10^(-22))。CCL20、PRMT1和UCK2基因表达与血清AFP有关,CCL20、PTTG1、PRMT1、TOP2A和UCK2基因表达与组织学Edmondson-Steiner分级有关,CCL20基因表达与TNM分期有关(均P<0.05)。结论筛选出HCC中表达上调最为明显的10个基因,将为HCC分子靶向治疗研究提供潜在靶点。

Objective To screen the most overexpressed gene panel in hepatocellular carcinoma（ HCC） using the gene expression profiles. Methods The global gene expression profiles were measured using Affymetrix U133 Plus 2. 0 array in 93 HCC and paired non-tumor liver tissues. Paired t-test was used to compare the gene expression levels between HCC and paired non-tumor tissues,and the chi-square test was used to analyze the relationship between the gene expression and clinicopathological characteristics.Results When compared with the paired non-tumor tissues,the top 10 overexpressed genes in HCC were CDKN3,CCL20,PTTG1,MELK,PRMT1,TOP2 A,TPX2,SMYD3,TMEM106 C and UCK2,with the upregulated folds between 25. 2 and 44. 6（ all P≤2. 0×10^-22）. High expression（ 〉median） of the CCL20,PRMT1 and UCK2 genes was significantly associated with high serum AFP levels.High expression of the CCL20,PTTG1,PRMT1,TOP2 A and UCK2 genes,was associated with high Edmondson-Steiner grade; and high CCL20 expression was also significantly associated with advanced TNM stage（ all P〈0. 05）. Conclusion The top 10 overexpressed genes in HCC is screened,which may provide the potential targets for the studies of molecular target therapy.