UPLC法同时测定淫羊藿中5种黄酮类成分的含量

Simultaneous Determination of Five Flavonoids in Epimedii Folium by UPLC

目的建立同步测定中药材淫羊藿中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷I 5种黄酮类成分含量的UPLC-DAD法。方法采用Aglientl290型超高效液相色谱仪,色谱柱为Aglient Zorlbax EclipsePlus C_(18)柱(50mm×2.1mm,1.8μm),柱温为40℃。流动相为乙腈和0.1%磷酸水,梯度洗脱,流速为0.4mL/min,进样量为1μL,检测波长为270nm。结果淫羊藿药材中5种黄酮类成分分别在2.02~202μg/mL、2.12~212μg/mL、2.14~214μg/mL、2.52~252μg/mL和1.12~112μg/mL的浓度范围内呈良好的线性关系,最低检测限分别为2.82、3.03、2.72、3.09、1.23ng/mL。重复性、稳定性试验结果的RSD值均小于3%,各成分平均回收率分别为99.76%、98.96%、98.76%、98.67%和98.65%。同时对14批不同药房或产地的药材中5种黄酮类成分的含量进行了检测。结论该方法快速简便、准确可靠,各主要化学成分均能达到基线分离,适用于淫羊藿药材的多成分分析,进而为淫羊藿药材质量控制的全面评价提供科学依据。

s： Objective To establish a UPLC-DAD method for simultaneous determination of the content of 5 flavonoids （epimedins A, B, C, icariin and baohuoside I） in Epimedii Folium. Methods Aglient 1290 ultra performance liquid chromatography was used, the column was Aglient Zorbax Eclipse Plus C18 （50mm× 2. 1 mm, 1.8μm）, column temperature was 40℃. Mobile phase composed of acetonitrile and 0.1% phosphate water with gradient elution at flow rate of 0.4mL/min, injection volumn was 1μL, detection wavelength was 270nm. Results The linear ranges of 5 flavonoids were 2.02-202μg/mL, 2.12-212μg/mL, 2.14-214μg/ml, 2.52-252 μg/mL and 1.12-112μg/mL respectively with good linear relationships. The lowest detection limits were 2.82, 3.03, 2.72, 3.09, 1.23ng/mL respectively. The RSD results of repeatability and stability were less than 3%. The average recovery rates were 99.76%, 98.96%, 98.76%, 98.67% and 98.65% respectively. The contents of 5 flavonoids from 14 different pharmacies of regions were also detected. Conclusion The method is rapid, simple, accurate and reliable, which can be used for quality control standard of Epimedii Folium.