激活蛋白-1（c-Fos／c-Jun）mRNA水平与牙龈炎症相关性研究

Correlation between the expression of activator protein-1 （c-Fos/c-Jun） mRNA and gingival inflammation

目的研究激活蛋白-1（AP-1）（c-Fos／c-Jun）mRNA的表达水平与牙龈炎症间的相关性，探讨牙周炎的发病机理。方法将获得的牙龈组织根据牙龈指数（GI）分为3组：GI=0组（对照组，14例）、GI=I组（15例）和GI=2组（11例）。提取各组牙龈组织的总RNA，反转录合成cDNA，采用反转录PCR检测健康牙龈组织（GI=0组）中c-FosmRNA和c-JunmRNA的表达情况，实时定量PCR分析各组c-FosmRNA和c-JunmRNA的表达水平。结果c—FosmRNA和c-JunmRNA在健康牙龈组织中均有表达。GI=1组c-FosmRNA和c-JunmRNA的表达水平（15．58±9．19、3．47±1．77）明显高于GI=0组（1．31±1．03、1．32±0．94），差异均具有统计学意义（均P〈0．05）。GI=2组c．FosmRNA的表达水平（3．01±1．48）低于GI=1组（P〈0．05），但高于GI=0组（P〈O．05）；c，JunmRNA的表达水平（1．48±0．65）低于GI=I组（P〈0．05），但与GI=0组间差异无统计学意义（P〉0．05）。结论AP-1（c-Fos／c—Jun）与牙龈炎症程度存在相关性，提示其参与牙龈炎症的发生发展。

Objective To study the correlation between the expression of activator protein-1 （c-Fos/c-Jun） mRNA and gingival inflammation, so as to discuss the pathogenesis of periodontitis. Methods The gingival tissues were divided into three groups according to the gingival index （GI）, including GI=0 group （control group, 14 cases）, GI=1 group （15 cases） and GI=2 group （11 cases）. The total RNA in each gingival tissue was extracted, and eDNA was synthesized by reverse transcription synthesis. The expressions of c-Fos and c-Jun mRNA in healthy gingival tissue （GI=0 group） were detected by reverse transcription-polymerase chain reaction. The levels of c-Fos and c-Jun mRNA in all the groups were detected by real-time quantitative PCR. Results Both c-Fos and c-Jun mRNA was expressed in healthy gingival tissues. The levels of c-Fos and c-Jun mRNA in GI=1 group was 15.58±9.19 and 3.47± 1.77, respectively, which was significantly higher than 1.31 ±1.03 and 1.32±0.94 in GI=0 group, and the differences were statistically significant （all P〈O.05）. The level of e-Fos mRNA in GI=2 group was 3.01±1.48, which was lower than that in GI=I group （P〈0.05） and higher than that in GI=0 group （P〈0.05）. The level of e-Jun mRNA in GI=2 group was 1.48±0.65, which was lower than that in GI=1 group, and had no significant difference with GI=0 group （P〉 0.05）. Conclusions Activator protein-1 （c-Fos/c-Jun） is associated with the degree of gingival inflammation, suggesting that it is involved in the occurrence and development of gingival inflammation.