ANGPTL5在人胃癌组织中的表达及临床意义

Expression and clinical significance of angiopoietin-like protein 5 in human gastric cancer tissues

目的探讨血管生成素样蛋白5(ANGPTL5)在人原发胃癌组织中的表达及与临床病理因素和预后的关系。方法收集73例胃癌组织及对应癌旁正常组织,通过免疫组织化学染色法检测ANGPTL5蛋白的表达。使用Pearsonχ~2检验和连续校正的χ~2检验法分析癌组织与癌旁正常组织中ANGPTL5蛋白的表达差异及ANGPTL5蛋白与临床病理因素和预后的关系。结果 ANGPTL5主要弥漫表达在胃癌细胞胞浆中,部分间质细胞中弱表达,癌旁正常组织ANGPTL5偶有表达。ANGPTL5在胃癌组织中的表达显著高于癌旁正常组织(χ~2=38.939,P<0.001)。在胃癌组织中ANGPTL5的表达水平与原发肿瘤大小、浸润深度和TNM分期显著相关(P<0.05)。ANGPTL5高表达组总生存期明显低于低表达组(χ~2=5.888,P=0.015)。结论 ANGPTL5在胃癌组织中高表达,且与胃癌预后不良因素及较短生存期相关,提示ANGPTL5可能参与胃癌的进展,是潜在的胃癌预后不良因子和治疗靶点。

Objective To investigate the expression of angiopoietin-like protein 5（ ANGPTL5） and its clinical significance in primary human gastric cancer（ GC）. Methods Using immunohistochemistry（ IHC）,the expression of ANGPTL5 protein in 73 human specimens was detected including GC tissues and adjacent normal tissues. Pearson Chisquare test and continuity correction for Pearson Chi-square test were used to analyze the difference of ANGPTL5 expression between cancer tissues and adjacent normal tissues and the association between clinical pathological parameters and ANGPTL5 expression in GC tissues. Results ANGPTL5 was mainly expressed in cytoplasm of GC cells,and faint staining was presented in parts of interstitial cells,but most of adjacent normal gastric cells had no or little ANGPTL5 expression. A significant overexpression of ANGPTL5 protein was presented in GC tissues compared with that in adjacent normal tissues（ χ~2= 38. 939,P 〈 0. 001）. Moreover,ANGPTL5 expression was significantly associated with primary tumor size（ χ~2= 5. 006,P = 0. 025）,depth of tumor infiltration（ χ~2= 6. 810,P = 0. 009） and TNMstages（χ~2= 4. 867,P = 0. 027）. Furthermore,the overall survival（ OS） in ANGPTL5 positive group was lower than that in the negative group（ χ~2= 5. 888,P = 0. 015）. Conclusion ANGPTL5 was highly expressed in GC tissues,which wasassociated with poorly prognostic factors and shorter OS,suggesting that ANGPTL5 may be involved in GC progression,and it might be a new potential poor prognostic indicator for GC treatment.