人工栽培北虫草中超氧化物歧化酶提取工艺的优化实验

Optimization in Extraction Process of Superoxide Dismutase from Artificial Cultivation of Cordyceps Militaris

目的:优选北虫草中SOD(SOD)的最佳提取工艺。方法:采用单因素分析,以北虫草SOD的含量为指标,考察北虫草中SOD提取条件,以及考察pH值、料-液比、提取时间对提取率的影响,从而确定北虫草SOD的最佳提取工艺。结果:建立了北虫草SOD的最佳提取工艺为磷酸盐缓冲液pH值为7.8、料-液比为1∶40,超声提取时间为60 min。结论:优化的提取工艺稳定、合理、可行,能正确反映北虫草药材中的SOD含量,为北虫草生物活性物质的进一步研究提供了实验依据。

Objective： To optimize the extraction process of Superoxide Dismutase（SOD） from artificial cultivation of Cordyceps Militaris. Methods： Based on the single factor analysis and taking the content of SOD as an index, the influence of the pH, solid-liquid ratio and extraction time on extraction yield are investigated. Results： The optimum extraction conditions of SOD were pH 7.8, 60min in a solid-liquid ratio of 1 and 40. Conclusion： The extraction process is stable, reasonable and feasible and it could correctly reflect the content of SOD in Cordyceps Militaris.