结节性甲状腺肿组织中Pendrin基因及蛋白表达的研究

Expression of Pendrin gene （SLC26A4） and protein in multinodular goiter

目的研究结节性甲状腺肿中Pendrin基因（SLC26A4）及蛋白的表达，探讨其与结节性甲状腺肿的关系。方法选取手术切除的结节性甲状腺肿40例（结节组），对应的正常甲状腺组织40例（对照组）．采用实时荧光定量聚合酶链反应法（realtime polymerase chain reaction，RT—PCR）检测2组SLC26A4基因表达．蛋白免疫印迹技术（western blot）及免疫组织化学方法检测2组Pendrin蛋白的表达及分布情况。结果结节组SLC26A4基因mRNA的水平较对照组mRNA的水平表达增高，差异具有统计学意义（t=2．663，P=0．011）；Pendrin蛋白在结节组水平高于对照组（t=2．286，P=0．026）。结节组Pendrin蛋白阳性表达24例（60％），对照组阳性表达14例（35％），差异有统计学意义（X2=5．013，P=O．025）。结节组中Pendrin蛋白主要在细胞质中．对照组则主要在细胞膜上。结论结节组中SLC26A4 mRNA及其编码Pendrin蛋白表达增强，且大部分蛋白表达分布在细胞质内，提示发生结节性甲状腺肿时，甲状腺碘转运的功能存在一定程度的损害。

Objective To explore the expression of the Pendrin gene （SLC26A4） and protein in multin-odular goiter. Methods Thyroid tissues were obtained from 40 muhinodular goiter patients undergoing surgery while the control group were obtained from 40 normal thyroid tissues. RT-PCR was used to test SLC26A4 gene while western blot and immunohistochemistry were used to test Pendrin protein expression and distribution. Results SLC26A4 mRNA expression in muhinodular goiter tissue was significantly increased in comparison with normal nodular tissues （t=2.663 ,P=0.011 ）. Pendrin protein expression in muhinodular goiter group was higher than that in normal tissue （t=2.286,P=0.026）. The immunohistochemistry results showed that the Pendrin protein in muhinodular goiter was mainly located in cytoplasm. There was positive expression in 24 patients （60%） in multin- odular goiter group, while it was in 14 patients （350/o） in the normal control group. The difference was significant （X2=5.013,P=0.025）. Pendrin protein mainly expressed in cytoplasm in multinodular goiter tissue while it was mainly in cytomembrane in the normal control group. Conclusion SLC26A4 mRNA and its coding protein Pen- drin expression are increased in multinodular goiter group, and mainly located in cytoplasm, indicating that iodide transporter function may be damaged when muhinodular goiter occurs.