摘要
目的探讨外周血CD；cD去调节性T（n）细胞和白细胞介素17（IL-17）水平在抗磷脂抗体综合征（APS）患者中的变化及其临床意义。方法采用流式细胞仪检测APS患者60例（APS组）外周血n细胞数量及占CD4^＋细胞比例，酶联免疫吸附测定法（ELISA）检测血浆中IL-17水平，并与对照组60例进行比较分析。结果APS组Tr、Tr／CD4^、Foxp3^＋Tr／Tr分别为（3．03±0．40）％、（0．11±0．02）、（0．40±0．10），均低于对照组的（5．76±0．84）％、（0．15±0．03）、（0．73±0．15），两组差异均有统计学意义（t=22．73、8．59、14．18，均P〈0．05）；APS组IL-17、IL-17／Tr分别为（15．31±2．00）pg／mL、（5．05±0．29）pg／mL，均高于对照组的（6．63±1．52）pg／mL、（1．15±0．14）g／mL，两组差异均有统计学意义（t=29．17、93．81，均P〈0．05）；APS组Tr水平与IL-17水平呈负相关（r=-0．801，P〈0．01）。结论APS患者存在免疫功能紊乱，CD4^＋CD58^＋Tr细胞数目变化、活性变化以及IL．17／Tr比值失衡可能在APS发病机制中起-定的作用。
Objective To explore the changes of Tr cells and IL - 17 levels in peripheral blood of patients with antiphospholipid syndrome （APS） and the clinical significance. Methods The quantity of the CD4^＋ CD^＋ Tr cells and proportion of CD4^＋ CD2^＋ Tr cells/CD4 T ceils were detected by flow cytomety, and the levels of IL - 17 were detected by ELISA in 60 cases with APS（ APS group） and 60 healthy people （control group）. Results The levels of Tr,Tr/CD4^＋ ,Foxp ＋ 3Tr/Tr in the APS group were （3.03 ＋0.40）% ,（0.11 ±0.02） ,（0.40 ±0.10） ,respectively, which in the control group were （5.76 ± 0.84） % , （0.15± 0.03 ） , （0.73 ± 0.15 ） , respectively, the differences were statistically significant（ t = 22.73,8.59,14.18, all P 〈 0.01 ）. The levels of serum IL - 17 and IL - 17/Tr cells in APS group were （ 15.31 ± 2.00） pg/mL, （ 5.05± 0.29 ） pg/mL, respectively, which in the control group were （ 6.63 ± 1.52 ） pg/mL, （ 1.15 ± 0.14 ） pg/mL, respectively, there were statistically significant differences （ t= 29.17,93.81, all P 〈0. 05 ）. The levels of serum IL - 17 was negatively correlated with Tr cells level in peripheral blood of patients with APS（r = -0. 801 ,P 〈0.01 ）. Conclusion There is peripheral blood cellular immune function disorder in APS patients ,the number and active changes of CD4^＋CD58^＋ Tr cells and IL - 17/Tr ratio imbalance may play a role in the pathogenesis of APS.
出处
《中国基层医药》
CAS
2017年第18期2737-2740,共4页
Chinese Journal of Primary Medicine and Pharmacy
