针灸对感染性休克患者CD4^＋CD25^＋调节性T细胞及Foxp3的影响

Effects of acupuncture on the ratio of CD4^＋CD25^＋ regulatory T cells and expression of transcription factor Foxp3 in patients with septic shock

目的研究针灸对感染性休克患者CD4^＋CD25^＋调节性T细胞及Foxp3的影响。方法将64例感染性休克患者采用随机数字表法分为针灸组34例和对照组30例，针灸组采用常规西医治疗联合针灸治疗，对照组采用常规西医治疗，7d后采用流式细胞术检测两组患者外周血CD4^＋CD25^＋Treg细胞的含量，灾时荧光定量PCR检测两组患者外周血Foxp3mRNA的表达。结果治疗7d后，对照组外周血cDfcD未Treg细胞、CD4^＋CD25^＋Foxp3＋Treg细胞比例分别为（20．23±1．12）％、（78．70±7．65）％，针灸组外周血CD4^＋CD25^＋Treg细胞、CD4^＋CD25^＋Foxp3＋Treg细胞比例分别为（17．32±0．78）％、（68．53±8．01）％，两组差异均有统计学意义（t=2．587、2．749，均P〈0．05）。治疗7d后，对照组、针灸组外周血中Foxp3mRNA的表达分别为（1．214-0．02）、（1．02±0．04），两组差异有统计学意义（t=2．119，P〈0．05）。结论针灸叮能是通过减少CD4^＋CD25^＋Treg细胞比例、下调Foxp3mRNA表达，凋节感染性休克患者的免疫状态。

Objective To study the effect of acupuncture on the ratio of CD4^±CD25^± regulatnoT T cells and expression of transcription factor Foxp3 in patients with septic shock. Methods Sixty - four patients with septic shock were randomly divided into two groups by using the random number table metbod. Acupuncture group （34 cases） was treated with both western medicine and acupuncture, and control grotJp （30 cases） was treated with western medicine. The period of treatment was 7 days. After treatment,the ratio of CD4^±CD25^± T cells in peripheral blood was determined by flow cytometl7. And the expression of Foxp3 mRNA in peripheral blood was detected by quantita- tive real time PCR. Results After treating for 7 days, the ratios of CD4^±CD25^± Treg cells and CD4^±CD25^± Fnxp3 ± Trcg cells were （ 20.23 ± 1.12） % and （78.70 ± 7.65 ） % respectively in peripheral blood of the control group, which in the acupuncture groupwere （ 17.32 ± 0.78 ） % and （ 68.53 ± 8.01 ） %, the differences were statistically significant between the two groups （ t = 2. 587,2. 749, all P 〈 0.05 ）. The levels of Foxp3 mRNA in peripheral blood were （ 1.21 ± 0.02） and （ 1.02 ±0.04） in the control group and acupuncture group, the difference was statistically significant（ t = 2.119,P 〈 0. 05）. Conclusion Acupuncture can adjust immune status of patients with septic shock by reducing the ratio of CD4^±CD25^± Treg cells and down - regulating the expression of Foxp3 mRNA.