同时测定小儿咳喘灵颗粒中4种成分的方法研究

Study on the Method for Simultaneous Determination of Four Components in Xiaoer Kechuanling Granules

目的:建立同时测定小儿咳喘灵颗粒中(R,S)-告依春、绿原酸、甘草苷和木犀草苷含量的方法。方法:采用高效液相色谱法,Inertsil C8-3色谱柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.5%乙酸溶液,梯度洗脱;流速:0.6 m L·min-1;DAD检测器,检测波长:木犀草苷327 nm、绿原酸327nm、甘草苷276 nm、(R,S)-告依春245 nm。结果:金银花中木犀草苷和绿原酸、甘草中甘草苷及板蓝根中(R,S)-告依春在各自的线性范围内与其峰面积呈良好的线性关系;平均加样回收率在99.4%~103.4%范围内,RSD均符合规定。结论:该方法准确、简便、快速,可用于小儿咳喘灵颗粒中多味药材的多个活性成分的含量测定。

Objective： To establish a method for the simultaneous determination of （R,S）-goitrin, chlorogenic acid, liquiritin and luteoloside in Xiaoer Kechuanling Granules. Methods： HPLC was conducted on a Dikma Inertsil C8-3 column （4.6 mm×250 mm, 5 μm） with the mobile phase of Acetonitrile and 0.5% acetic acid at a flow rate of 0.6 mL · min^-1 under gradient elution. Detection wavelengths of DAD detector were as follows： luteoloside （327 nm）, chlorogenic acid （327 nm）, liquiritin （276 nm）, as well as （R, S）- goitrin （245 nm）. Results： Luteoloside and chlorogenic acid in honeysuckle, liquiritin in liquorice, and （R, S）- goitrin in radix isatidis all had a good linear relationship with their peak area. The average recoveries were in the range of 99.4% - 103.4%. RSD was in accordance with the regulations. Conclusion： The method is accurate, simple, and rapid, and it can be used in the determination of multiple medicinal materials and active components in Xiaoer Kechuanling Granules.