摘要
目的:从不同角度对人乳牙牙髓干细胞(SHED)与恒牙牙髓干细胞(DPSCs)的生物学功能进行对比研究,探讨对骨改建的影响。方法:有限稀释法分离培养SHED和DPSCs,成脂诱导14d进行油红O染色、成骨诱导21d进行茜素红染色;ELISA法检测IL-6、TNF-α分泌水平;两种干细胞常规培养及成骨诱导7d后进行ALP染色、实时定量PCR检测Runx2、RANKL、OPG基因表达。结果:分离获得的SHED和DPSCs具有成骨、成脂分化能力,符合干细胞特点。SHED的炎性细胞因子IL-6、TNF-α的表达均高于DPSCs(P<0.01);成骨诱导7d后SHED的ALP活性强于DPSCs(P<0.05);且经成骨诱导后两种干细胞均表达Runx2、OPG和RANKL,但SHED的表达水平明显高于DPSCs(P<0.05)。结论:SHED与DPSCs相比不仅具有较强的成骨分化能力,而且兼具较强的破骨能力,提示SHED可能参与骨改建调控机制。
Objective: Research differences of biological functions between stem cells from human exfoliated deciduous teeth and dental pulp stem cells from different angles,to explore the influence of bone reconstruction. Methods: SHED and DPSCs were isolated, purified and cultured in vitro by limiting diluction, and oil red O staining at 14 days of adipogenic induction,alizarin red staining at 21 days of osteogenic induction; detect IL-6 and TNF-o~ secretion level by Elisa method; ALP staining at 7 days of two kinds of stem cells in conventional cultivation and osteogenic induced, and detect Runx2, RANKL and OPG gene expression by real-time PCR. Results: Both of SHED and DPSCs have abilty to adipogenic and osteogenic differentiation; conforms to the characteristics of the stem cells. SHED expression of IL-6 and TNF-ct were higher than that of DPSCs(P〈 0.05); Both kinds of stem cells express Runx20PG and RANKL after osteoinductive differentiation, but the expression of SHED obviously higher than that of DPSCs(P〈0.05). Conclusion: Compared with DPSCs, SHED has not only the ability of osteogenic differentiation but also an osteoclast capacity,remind that SHED may participate in the bone reconstruction.
出处
《中华老年口腔医学杂志》
2017年第4期204-208,共5页
Chinese Journal of Geriatric Dentistry