一氧化氮清除剂Fe(DETC)_2对小鼠的辐射防护作用

Radioprotective effects of a nitric oxide scavenger, Fe(DETC)_2, on mice

采用小鼠30 d存活率实验和外周血白细胞、骨髓DNA含量、脾克隆形成单位及各脏器指数指标考察NO特异性清除剂二乙基二硫代氨基甲酸亚铁(Fe(DETC)2)对辐射损伤小鼠的保护作用。结果显示,与单纯照射组相比,给予不同剂量Fe(DETC)2后,照射给药组小鼠30 d存活率、外周血白细胞数、骨髓DNA含量、脾克隆形成单位及各脏器指数均有不同程度的提高,特别是二乙基二硫代氨基甲酸钠(DETC)以300 mg/kg的剂量腹腔注射,可使存活率提高约43%,白细胞数、骨髓DNA含量和脾克隆形成单位与单纯照射组相比具有统计学意义(p<0.01)。结果提示,一氧化氮清除剂Fe(DETC)2对辐射损伤小鼠具有一定的保护作用。

A 30-day survival rate experiment was carried out, and the assays of peripheral blood leukocytes, bone marrow DNA content, colony forming units of the spleen（CFU-S）, and organ index parameters were analyzed to evaluate the protective effect of a nitric oxide-specific scavenger, Fe-（2＋）-diethyldithiocarbamate complex（Fe（DETC）2）, on radiation-injured mice. The results showed that compared with the irradiation group, the 30 d survival rate, peripheral blood leukocytes, bone marrow DNA content, CFU-S, and organ index were significantly different from those in the mice treated with different doses. In particular, DETC was administered intraperitoneally at a dose of 300 mg/kg, which increased the survival rate by about 43%, and changes in the number of white blood cells, bone marrow DNA content, and CFU-S were statistically significant（P〈0.01）. It is suggested that Fe（DETC）2, a nitric oxide scavenger, has a protective effect on radiation-injured mice.