高效液相色谱法测定芩倍合剂中黄芩苷、柚皮苷含量

Determination of baicalin and naringin inQinbei mixture by HPLC

目的 建立芩倍合剂中黄芩苷、柚皮苷的含量测定方法,确定其含量测定指标成分及其限量标准.方法 色谱柱为Diamonsil C18（4.6 mm×250 mm,5μm）,流动相为甲醇：0.4%磷酸水溶液（42：58）,流速1.0 ml/min,检测波长280 nm,柱温30℃,进样量10μl.结果 黄芩苷、柚皮苷分别在0.062~0.930μg、0.033~0.492μg范围内线性关系良好,平均加样回收率分别为98.11%（RSD=1.62%）、96.78%（RSD=1.74%）.根据4批样品的含量测定结果,确定每1 ml芩倍合剂中黄芩苷平均含量为8.4 mg,柚皮苷平均含量为0.5 mg.结论 该方法简便、灵敏、准确、重现性好,适用于芩倍合剂中有效成分的含量测定.

Objective To establish a high performance liquid chromatography （HPLC） method for the determination of baicalin and naringin inQinbei mixture.Methods The HPLC system consisted of the Fortis-C18（4.6 mm × 250 mm, 5μm） column, and the mobile phase consisted of MeOH：0.4% H3PO4 （42：58）, and the flow rate was 1.0 ml/min, and the UV detector was set at 280 nm, and the column temperature was 30℃.Results The linear response range of baicalin was 0.062-0.930μg. The linear response range of naringin was 0.033-0.492μg. The average recovery of baicalin was 98.11% （RSD=1.62%）. The average recovery of naringin was 96.78% （RSD=1.74%）.Conclusions The method is simple, rapid, accurate and repeatable. It can be applied in determination of baicalin and naringin inQinbei mixture.