PTC-209靶向抑制Bmi1对人舌鳞癌细胞的体外抗癌作用

Therapeutic targeting of Bmi1 with PTC-209 in tongue squamous cell carcinoma in vitro

目的:检测Bmi1(B lymphoma Mo-MLV insertion region 1)小分子化学抑制剂PTC-209对人舌鳞癌细胞中Bmi1表达的抑制作用,并探讨其对体外人舌鳞癌细胞生物表型的影响。方法:对人舌鳞癌细胞系Cal27予以PTC-209处理,通过蛋白质免疫印迹、实时定量逆转录聚合酶链反应检测PTC-209干预后细胞内Bmi1的表达变化;通过MTT、Transwell、划痕试验等分析PTC-209处理对人舌鳞癌细胞增殖、迁移和侵袭的影响;应用克隆形成、肿瘤球培养、流式分选等实验分析PTC-209对人舌鳞癌细胞系中肿瘤干细胞亚群的影响。结果:PTC-209能显著下调人舌鳞癌细胞中Bmi1的表达,并具有浓度和时间依赖效应(P<0.05);PTC-209体外干预可明显抑制细胞增殖、侵袭和迁移能力,增强细胞对顺铂和5-FU的药物敏感性;PTC-209能显著降低细胞克隆形成率、肿瘤球形成以及ALDH+亚群细胞比例。结论:PTC-209能在体外抑制人舌鳞癌细胞中Bmi1的表达,具有抑制细胞增殖、侵袭迁移和降低肿瘤干细胞亚群比例等抗肿瘤效应。

Objective：In the present study,we aimed to investigate the small-molecule B lymphoma Mo-MLV insertion region 1 （Broil） inhibitor,PTC-209,for its effects on endogenous Bmil expression and cell behaviors in tongue squamous cell carcinoma （TSCC） cell line in vitro. Methods：The TSCC cell line Cal27 was treated with PTC-209 in vitro ,and the expression change of Broil was detected by Western blotting and real-time RT-PCR assays. The changes of cell proliferation,invasion and migration were ana- lyzed by MTT, transwell and wound-healing assay, respectivety. The cancer stem cell （CSC） subpopulations in Cal27 were further as- sayed by tumorsphere formation,colony-forming and flow cytometry assays. Results ：Bmil mRNA and protein levels were significantly decreased in Cal27 cells treated with PTC-209 in both dose- and time-dependent manners （P〈0.05）. PTC-209 inhibited cell prolifera- tion ,migration and invasion ,and had synergic anti-cancer effects when combined with cisplatin and 5-FU. Moreover,colony forma- tion, sphere formation, and ALDH＋ subpopulation were significantly reduced upon PTC-209 exposure. Conclusion：PTC-209 treatment induces Bmil reduction in TSCC cell line in vitro ,and shows potent anti-tumor activity with reduced cell proliferation ,migration ,in- vasion and CSC subpopulation.