兴国灰鹅微卫星标记的遗传多样性分析

Genetic diversity analysis of Xingguo gray goose based on microsatellite markers

为了解兴国灰鹅的遗传多样性,揭示其群体遗传结构,利用10个微卫星标记对兴国灰鹅进行哈代-温伯格平衡(HW)检验,统计群体基因频率、观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)、等位基因(Na)和有效等位基因(Ne)等。结果显示:10个微卫星标记位点共检测到30个Na,其中Ne为20.549个;Ho、He及PIC值最高的标记为CKW21,分别为0.700、0.734 5、0.679 5;群体平均Ho为0.384、He为0.477 5,平均PIC为0.411 1,表明兴国灰鹅遗传多样性丰富;经HW检验,兴国灰鹅在10个标记位点中存在3个不平衡点,表明群体的遗传结构处于一个逐渐不平衡的状态。

In order to understand the genetic diversity of Xingguo gray goose and reveal the genetic structure of its population,ten microsatellite markers were selected for Hardy-Weinberg equilibrium test. The genetic diversity was disclosed by calculating the allele frequency,observed heterozygosity（ Ho）,expected heterozygosity（ He）,effective number of alleles and polymorphism information content（ PIC）. The results indicated that a total of 30 alleles were detected in all of the 10 microsatellite markers. The most polymorphic marker was CKW21,which the observed heterozygosity,expected heterozygosity and polymorphic information content were 0. 700,0. 734 5 and 0. 679 5,respectively. In addition,the average observed and expected heterozygosities were 0. 384 0 and 0. 477 5,respectively,the average polymorphism was 0. 411 1. Our results suggested that Xiongguo gray goose accumulated abundant genetic diversity. There were three unbalanced loci out of ten marker loci in Xiongguo gray goose by the Hardy-Weinberg equilibrium test and showed that the genetic structure of the group was in an unbalanced state gradually.