1例红颊竹鼠化脓性肺炎病原菌的分离鉴定

Isolation and identification of a pathogenic bacterium from bamboo rats with abscessing pneumonia

为确定南宁金陵镇某竹鼠养殖场发生竹鼠化脓性肺炎的原因,从发病竹鼠肺中分离得到可疑细菌,经形态观察、生理生化特性测定及16S rRNA序列分析,鉴定该分离菌为大肠杆菌,经小鼠回归试验证实该分离菌可致死小鼠。该菌16S rRNA与大肠杆菌(DQ337503,FJ418574,KR189581)同源性高达100%。药敏试验显示对头孢克肟、卡那霉素、大观霉素、奈替米星、氟苯尼考和米诺环素等药物高度敏感,对氨苄西林、阿莫西林、亚胺培南、头孢唑啉、头孢氨苄、四环素、红霉素和克拉霉素等药物有抗性。结果表明:大肠杆菌是南宁金陵镇某竹鼠养殖竹鼠致死的病原菌。该菌的分离鉴定为其感染引起的传染病防治提供了试验依据。

This study aimed to determine the pathogen infecting the bamboo rats with abscessing pneumonia in Jinling town of Nanning. One bacterial strain was isolated from the lungs of diseased bamboo rats. The analysis by morphological characteristic,physiological and biochemical assays,and 16S rRNA gene sequence demonstrated that the pathogenic bacterium was Escherichia coli. The virulence assay showed that the isolate was highly pathogenic to the adult mice. The 16S rRNA gene sequence closely related to Escherichia coli（ DQ337503,FJ418574,KR189581） with homologies of 100%. The susceptibility test to antibiotic demonstrated that the bacterial strain was highly sensitive to cefixime,kanamycin,spectinomycin,netilmicin,florfenicol and ninocycline,but resistant to ampicillin,amoxicillin,imipenem,cefazolin,cephalexin,tetracycline,erythromycin and clarithromycin. The study provides a basis for disease control of the bamboo rats in Jinling town of Nanning.