MicroRNA生物合成通路中Gemin 3基因单核苷酸多态性与非霍奇金淋巴瘤风险及预后相关性

Correlation of Gemin 3 SNP in the microRNA Biosynthesis Pathway with Risk and Prognosis of Patients with Non-Hodgkin's Lymphoma

目的:评估miRNA生物合成过程中Gemin 3基因位点rs197412的单核苷酸多态性与患非霍奇金淋巴瘤(NHL)风险及预后的相关性。方法:在230名NHL患者组和120名健康对照者组中采用聚合酶链反应-连接酶检测反应进行MiR-SNP基因分型。利用Kaplan-Meier法计算患者生存曲线,曲线之间的比较采用对数秩检验。多因素生存分析采用Cox比例风险模型进行。结果:rs197412基因分型在NHL组及对照组中分布无差异。患者携带rs197412 TT基因型比患者携带CC+CT基因型生存时间长(P=0.007)。此外,该rs197412 SNP也被确定为NHL预后的独立预测因子(相对危险度:2.138;95%可信区间:1.303-3.508;P=0.003)。结论:miRNA生物合成通路中Gemin 3基因位点rs197412的单核苷酸多态性与患NHL风险无关,但可能影响NHL的生存。

Objective： To evaluate the correlation of single nucleotide polymorphisms SNP） of Gemin 3（ rs197412）in the miRNA biosynthesis with NHL cancer risk and overall prognosis. Methods： miR-SNP were genotyped using PCRligase detection reaction（ LAR,LCR） in NHL group of 230 non-Hodgkin lymphoma（ NHL） patients and in control group of 120 healthy persons. The survival curves were drawn using the Kaplan-Meier method,and comparisons between the curves were made using the log-rank test. Multivariate survival analysis was performed by using a Cox proportional hazards model. Results： The rs197412 genotype distribution difference was not statistically significant,in NHL and control group; the survival time of patients carrying the rs197412 TT genotype was significantly longer than that of the patients carrying the CC ＋ CT genotype（ P = 0. 007）. In addition,rs197412 was independent from the survival of NHL patients by multivariate analysis（ RR： 2. 138, 95% CI： 1. 303-3. 508,P〈0. 01）. Conclusion： The single nucleotide polymorphisms of Gemin 3（ rs197412） in the miRNA processing are not related with NHL risk,but that may affect NHL survival.