捏脊对颈椎病大鼠椎间盘细胞外基质及代谢酶的影响

Effects of Chiropractics on Intervertebral Disk Extracell Matrix and Metabolic Enzymes in Rats with Cervical Spondylosis

目的:观察捏脊对颈椎病大鼠椎间盘基质金属蛋白酶(MMP)-1、MMP-3、MMP-13、细胞内Ⅱ型胶原(COL-Ⅱ)、基质金属蛋白酶抑制因子1(TIMP-1)以及细胞外基质(ECM)的影响,探讨捏脊治疗颈椎病的可能机制。方法:SD大鼠随机分为假手术组、模型组、捏脊组、莫比可组,每组8只。采用动静力失衡法建立颈椎病大鼠模型。捏脊组予捏脊干预治疗,每天14次;莫比可组予莫比可0.78mg/kg灌胃干预,每日1次,14d为1个疗程,共2个疗程。免疫组化法检测大鼠椎间盘组织中MMP-1、MMP-3、MMP-13、COL-Ⅱ、TIMP-1的表达量,免疫印迹法检测ECM的蛋白表达水平。结果:与假手术组比较,模型组大鼠椎间盘组织中MMP-1、MMP-3、MMP-13表达量均升高(P<0.05),COL-Ⅱ、TIMP-1表达均降低(P<0.05);与模型组比较,捏脊组与莫比可组大鼠椎间盘组织中MMP-1、MMP-3、MMP-13表达均降低(P<0.05),COL-Ⅱ、TIMP-1表达均升高(P<0.05)。与假手术组比较,模型组大鼠椎间盘组织中ECM蛋白表达降低(P<0.05);与模型组比较,捏脊组与莫比可组ECM蛋白表达升高(P<0.05)。结论:捏脊治疗颈椎病大鼠椎间盘退变可能是通过调节椎间盘ECM系统及相关代谢酶表达量实现的。

Objective To observe the effect of chiropractics on intervertebral disk extracell matrix metalloproteinase（MMP）-1,MMP-3,MMP-13,cellular collagen typeⅡ（COL-Ⅱ）,tissue inhibitor 1 of metalloproteinase（TIMP-1）and extracellular matrix（ECM）in the rats with cervical spondylosis,so as to explore its mechanism underlying improvement of cervical spondylosis.Methods A total of 40 SD rats were randomized into a sham group,a model group,a chiropractics group and a mobic group,8rats in each one.The model was established by static-dynamic imbalance method.The rats in the chiropractics group were treated with chiropractic therapy for two courses,14 times a day and 14 days as a course.The rats in the mobic group were treated with intragastric administration of mobic.The expression of MMP-1,MMP-3,MMP-13,COL-Ⅱ and TIMP-1 in the intervertebral disk tissue were detected by immunohistochemistry,and the protein expression of ECM was detected by Western blot.Results Compared with those in the sham group,the expressions of MMP-1,MMP-3,MMP-13 in the model group increased（all P〈0.05）,and COL-Ⅱ,TIMP-1 decreased（both P〈0.05）.Compared with those in the model group,the expressions of MMP-1,MMP-3,MMP-13 in the chiropractics group and the mobic group decreased（all P〈0.05）,and COL-Ⅱ,TIMP-1 increased（all P〈0.05）.Compared with that in the sham group,the expression of ECM protein in the model group decreased（P〈0.05）.Compared with that in the model group,the expression of ECM protein in the chiropractics group and the mobic group increased（both P〈0.05）.Conclusion Chiropractics may improve the degeneration in rats with cervical spondylosis by regulating intervertebral disc ECM system and relevant metabolic enzymes.