LPS调控人牙龈成纤维细胞表达uPA的研究

LPS induced urokinase-type plasminogen activator expression by human gingival fibroblasts

目的 :观察LPS对牙龈成纤维细胞表达尿激酶型纤溶酶原激活剂 (uPA)的影响。方法 :采用Westernblotting和Northernblotting分别观察LPS对牙龈成纤维细胞内uPA蛋白质表达水平和mRNA表达水平的影响。结果 :经 1.0 μg/mL LPS处理 8h后 ,牙龈成纤维细胞内uPA蛋白表达量明显增强 ,且这一增强作用可持续 2 4h ;经 1.0 μg/mLLPS处理 4h后 ,牙龈成纤维细胞内uPAmRNA表达量明显增强。 结论 :LPS能够促进牙龈成纤维细胞表达uPA ,参与牙周组织的破坏。

AIM: To investigate the effect of LPS on uPA expression by human gingival fibroblasts. METHODS: Western blotting and Northern blotting were used to observe the uPA expression by human gingival fibroblasts, which were stimulated by 1.0 μg/mL LPS. RESULTS: Human gingival fibroblasts had endogenous uPA expression, and the expression of uPA was increased significantly after stimulated with LPS for 8 h. The increased level of uPA expression was maintained for at least 24 h. uPA mRNA was increased after stimulated with LPS for 4 h. CONCLUSION: LPS induces uPA expression by human gingival fibroblasts.