1,25(OH)_2VitD_3对阿霉素肾病大鼠的保护作用

Protective effects of 1,25(OH)_2VitD_3 on adriamycin-induced nephropathy in rats

目的观察1,25(OH)_2VitD_3对阿霉素肾病大鼠的保护作用。方法将雄性SD大鼠随机分为正常组、模型组、药物组和阳性对照组,每组10只。模型组、药物组和阳性对照组大鼠尾静脉注射阿霉素制造慢性肾病模型。药物组和阳性对照组大鼠分别用5μg/(kg·d)^(-1)的1,25(OH)_2VitD_3和4 mg/(kg·d)^(-1)的贝那普利灌胃,正常组和模型组给予等量花生油灌胃。8周后检测大鼠24 h尿蛋白定量、血清尿素氮(blood urea nitrogen,BUN)、血肌酐(creatinine,Scr)、总蛋白(plasma total protein,TP)、白蛋白(serum albumin,ALB)、胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)的含量。统计采用单因素方差分析,组内比较采用LSD-t进行分析,以P<0.05为差异有统计学意义。结果模型组大鼠尿蛋白、BUN、Scr、TG、TC含量分别(106.9±7.19)mg/24 h、(41.73±4.26)mmol/L、(141.13±8.04)μmol/L、(3.27±0.15)、(9.41±0.33)mmol/L,较正常组的(10.9±4.22)mg/24 h、(8.58±3.55)mmol/L、(53.33±7.67)μmol/L、(0.37±0.14)、(1.46±0.24)mmol/L明显增多,差异均有统计学意义(均P<0.05),而TP、ALB含量分别为(42.80±4.74)、(19.36±2.44)g/L,较正常组的(74.48±2.68)、(34.27±2.00)g/L显著降低,差异均有统计学意义(均P<0.05)。药物组和阳性对照组干预治疗后,尿蛋白、BUN、Scr、TG、TC含量分别为(53.8±6.64)mg/24 h、(20.74±4.17)mmol/L、(77.58±7.94)μmol/L、(0.65±0.10)、(2.79±0.38)mmol/L和(56.8±4.59)mg/24 h、(23.12±4.65)mmol/L、(84.55±7.56)μmol/L、(0.75±0.21)、(3.08±0.46)mmol/L,较模型组明显降低,差异均有统计学意义(均P<0.05),而TP、ALB含量分别为(54.08±3.47)、(25.13±1.83)g/L和(49.15±4.36)、(23.51±1.82)g/L,含量显著增加,差异均有统计学意义(P<0.05)。结论 1,25(OH)_2VitD_3能减少尿蛋白的排出,提高血浆蛋白的含量,改善脂类代谢,对肾脏具有保护作用。

Objective To investigate the protective effects of 1,25（OH） 2VitD3 on adriamycin nephropathy in rats. Methods Male SD rats were randomly divided into four groups： the normal group, model group, drug group and positive control group, 10 rats in each group. Chronic renal disease model rats were established by intravenous injection of adriamycin in model group, drug group and positive control group. The rats of drug group and positive control group were treated by intragastric administration of 1,25（OH） 2VitD3 [5 礸/（kg穌）^（-1）] or benazepril [4mg/（kg穌）^（-1）]respectively for 8 weeks, meantime, the normal group and model group were given the same amount of peanut oil. At the 8 weeks, we observed the 24-hour urine protein, triglycerides（TG）, total cholesterol（TC）, plasma total protein（TP）,serum albumin（ALB）, blood urea nitrogen（BUN） and creatinine（Scr） levels. Results The 24-hour urine protein, BUN, Scr, TG, TC in model group were respectively（106.9？.19）mg/24 h,（41.73？.26）mmol/L,（141.13？.04）祄ol/L,（3.27？.15）,（9.41？.33）mmol/L, which were incresed remarkably compared with the normal group（10.9？.22）mg/24 h,（8.58？.55）mmol/L,（53.33？.67）祄ol/L,（0.37？.14）,（1.46？.24）mmol/L（P〈0.05）. While the conten of TP and ALB in model group were（42.80？.74）,（19.36？.44）g/L,and in normal were（74.48？.68）,（34.27？.00）g/L, there were statistically significan（P〈0.05）.The 24-hour urine protein, BUN, Scr, TG, TC in drug group and positive control group were respectively（53.8？.64）mg/24 h,（20.74？.17）mmol/L,（77.58？.94）祄ol/L,（0.65？.10）,（2.79？.38）mmol/L and（56.8？.59）mg/24 h,（23.12？.65）mmol/L,（84.55？.56）祄ol/L,（0.75？.21）,（3.08？.46）mmol/l, which were reduced remarkably compared with the model group（P〈0.05）. And the conten of TP and ALB in drug group and positive control group were respectively（54.08？.47）,（25.13？.83）g/L and（49.15？.36）,（23.51？.82）g/L, which were increased than that in model group（P〈0.05）.Conclusion 1,25（OH） 2VitD3 can significantly reduce urinary protein, increase plasma protein, improve lipid metabolism disorders, and has a protective effect on renal function.