3.0T磁共振质子波谱成像评价吉西他滨联合黄芪多糖注射液治疗胰腺癌模型兔的研究

Treatment of Pancreatic Cancer Model Rabbits by Docetaxel Combined with Astragalus Polysaccharide Injection Evaluated by 3.0T Proton Magnetic Resonance Spectroscopy

目的观察3.0T磁共振质子波谱成像评价吉西他滨联合黄芪多糖注射液治疗胰腺癌模型的效果。方法选取家兔60只成功制备胰腺癌模型,采用随机数字表法分为治疗1组、治疗2组及对照组,每组20只。治疗1组家兔给予尾静脉注射多西他滨联合黄芪多糖注射液,治疗2组家兔给予尾静脉注射多西他滨注射液,对照组家兔同期给予生理盐水等量注射,共治疗2周。对3组家兔同时行3.0T磁共振质子波谱成像检查,选择单体素点分辨波谱(PRESS)序列,分析波谱成像中代谢物的表现,比较3组家兔代谢物峰显示率并计算胆固醇与不饱和脂肪酸的混合峰(Chol+Unsat)与脂肪峰(Lip)峰高的比值(Chol+Unsat/Lip)。结果 3组家兔磁共振质子波谱成像主要代谢峰包括Lip峰、Chol+Unsat峰及胆碱峰(Cho),3组家兔Cho峰显示率比较,差异无统计学意义(P>0.05);对照组(Chol+Unsat)/Lip值为0.316±0.125,治疗1组(Chol+Unsat)/Lip值为0.224±0.095,治疗2组(Chol+Unsat)/Lip值为0.267±0.101,治疗1组、治疗2组分别与对照组比较,差异均有统计学意义(P<0.05),治疗1组与治疗2组比较,差异亦有统计学意义(P<0.05)。结论吉西他滨联合黄芪多糖注射液对胰腺癌模型具有较好的治疗效果,采用3.0T磁共振质子波谱成像可通过对代谢物波谱表现等指标评估胰腺癌模型的治疗效果。

Objective To observe the evaluation of 3. 0T magnetic resonance spectroscopy imaging（~1H MRS） for clinical efficacy of treating pancreatic cancer by docetaxel combined with Astragalus Polysaccharide Injection（ API）. Methods Recruited were 60 rabbits in which pancreatic cancer model were successfully prepared. Then they were divided into treatment group 1,treatment group 2,and the control group by random number table,20 in each group. Rabbits in treatment group 1were injected with docetaxel and API from tail vein. Rabbits in treatment group 2 were injected with gemcitabine from tail vein.Rabbits in the control group were injected with equal volume of normal saline. All rabbits were treated for 2 weeks. Rabbits received 3. 0T magnetic resonance proton spectroscopy at the same time. Proton spectroscopy sequence（ PRESS） was discriminated by selecting singular point. The performance of metabolites in spectral imaging was analyzed.（ Chol ＋ Unsat/Lip） of the mixed peak of cholesterol and unsaturated fatty acids（ Chol ＋ Unsat） and the peak of fat（ LIP） were calculated and compared among the three groups. Results The main metabolic peaks in the magnetic resonance proton spectroscopy of the three groupsmainly included Lip peak,Chol ＋ Unsat peak and choline（ Cho） peak. There was no significant difference in Cho peak rate among the three groups（P〈0. 05）. The mean value of（ Chol ＋ Unsat）/Lip was（ 0. 316 ± 0. 125） in the control group,（ 0. 224 ±0. 095） in treatment group 1,and（ 0. 267 ± 0. 101） in treatment group 2. Statistical difference existed between treatment group 1/2 and the control group（P〈0. 05） as well as between treatment group 1 and treatment group 2（P〈0. 05）.Conclusions Gemcitabine combined with API had favorable curative effect on pancreatic cancer. The therapeutic effect on pancreatic cancer model could be evaluated by performance of metabolite using 3. 0T magnetic resonance proton spectroscopy.