微小RNA-23a对乳腺癌细胞增殖和侵袭的影响

Effect of microRNA-23a on proliferation and invasion in breast cancer cells

目的研究微小RNA-23a(miR-23a)对乳腺癌细胞增殖和侵袭的影响。方法将人乳腺癌MCF-7和MDA-MB-435S复苏培养后,接种至6孔板中,随机分组进行培养,共分为6组:RNAi-Ⅰ组、过表达-Ⅰ组、对照-Ⅰ组、RNAi-Ⅱ组、过表达-Ⅱ组、对照-Ⅱ组。构建MiR-23a重组干扰及过表达载体,用Lipofectamine 2000将构建成功的MiR-23a重组干扰及过表达载体分别转染至各组人乳腺癌MCF-7和MDA-MB-435S细胞系中。结果在培养72 h后的OD492值:过表达组的MCF-7细胞和MDA-MB-435S细胞可分别达到0.52±0.03和0.44±0.03,而RNAi组MCF-7细胞和MDA-MB-435S细胞仅为0.36±0.02和0.31±0.02,与对照-Ⅰ组和对照-Ⅱ组比较,RNAi-Ⅰ组和RNAi-Ⅱ组的差异均有统计学意义(均P<0.05)。过表达-Ⅰ组和过表达-Ⅱ组的细胞倍增时间分别为(26.61±5.23),(29.23±5.51)h,而RNAi-Ⅰ组和RNAi-Ⅱ组的细胞则分别为(42.24±3.47),(58.17±5.34)h。过表达-Ⅰ组和过表达-Ⅱ组的M期细胞比例分别为52.36%,51.59%;而RNAi-Ⅰ组和RNAi-Ⅱ组则分别为76.70%,74.17%。与对照-Ⅰ组比较,RNAi-Ⅰ组和过表达-Ⅰ组的细胞倍增时间及M期比例差异均有统计学意义(均P<0.05)。结论 MiR-23a的表达水平与乳腺癌细胞的增殖和侵袭呈现正相关性。

Objective To investigate the effect of microRNA -23a （miR-23a） on proliferation and invasion of breast cancer cells. Methods Recombinant human breast cancer MCF - 7 and MDA-MB-435S were inoculated into 6-well plates and randomly divided into six groups, RNAi Ⅰ group, over expression - Ⅰ group, control Ⅰ group, RNAi Ⅱ group, over expression Ⅱgroup, controlⅡ group. Construct miR -23a recombinant interference and over expression vector. That were transfected into MCF- 7 and MDA -MB -435S cell lines with lipofeetamine 2000. Results OD492 value of MCF - 7 cells and MDA -MB -435S cells after 72 h culture： in over expression group could reach 0. 52 ± 0. 03 and 0. 44 ± 0. 03 respectively while they in RNAi group were only 0. 36 ± 0.02 and 0. 31 ± 0.02. Compared with control Ⅰ group and control Ⅱ group, RNAi- I group and RNAi - Ⅱ group had significantly different （all P 〈 0. 05 ）. The doubling time in over expression - Ⅰ and ]I groups were （ 26. 61 ± 5.23 ） , （29.23±5.51） h, respectively, while those in RNAi - Ⅰ and RNAi- Ⅱ groups were （42.24 ＋ 3.47）, （58. 17 ±5.34） h. The proportion of M phase cells in the over expression Ⅰ and Ⅱ groups were 52. 36% ,51.59%, respectively, while those in RNAi - Ⅰ and RNAi - Ⅱ groups were 76. 70% ,74. 17%. Compared with control Ⅰ group, the doubling time and proportion of M phase ceils in RNAi - Ⅰ group and over expression - Ⅰ group with significantly （ all P 〈 0. 05 ）. Compared with control Ⅱ group, the doubling time and proportion of M phase cells in RNAi - Ⅱ group andover expression - Ⅱ group with significantly （all P 〈 0. 05）. Conclusion The expression levels of miR - 23a showed a positive correlation with proliferation and invasion of breast cancer cells.