基于生物热动力学的黄连不同提取部分抑菌活性分析

Antibacterial Activities of Different Extracts from Coptidis Rhizoma Based on Biothermodynamics

目的:从生物热力学角度,探讨黄连不同提取部分抑菌作用差异,为黄连活性成分的研究提供新思路和理论依据。方法:采用微量量热法,在LB培养基,37℃条件下,接种量为1×10~5个/mL无菌条件下,建立大肠埃希菌、金黄色葡萄球菌和志贺氏痢疾杆菌的生物热动力学方法。利用该方法,绘制了3种菌分别在黄连水提物、生物碱部分和非生物碱部分作用下的生长热谱图(P-t图),得到相应的生物热动力学参数(主要包括生长速率常数k,细菌生长抑制率I,半数药物浓度抑制率IC_(50),最大产热功率P_(max)和细菌整达到P_(max)的时间t_(max)),综合分析不同提取部分对3种菌生长代谢作用差异。结果:黄连水提物和生物碱部分对3种菌生长有较强抑制作用,抑制顺序均为生物碱部分>水提物,而黄连非生物碱部分对3种菌生长不产生抑制作用,甚至有助菌生长的作用。同一提取部分对不同菌抑制作用顺序均为金黄色葡萄球菌>志贺氏痢疾杆菌>大肠埃希菌。结论:通过微量量热法实验结果分析,黄连的活性成分为生物碱部分,对金黄色葡萄球菌的生长抑制能力最强。

Objective：To explore the antibacterial activity differences of various extracts of Coptidis Rhizoma from the perspective of biothermodynamics,and to provide a new idea and evidence for the study on active components of Coptidis Rhizoma.Method：Microcalorimetry was used to establish biothermodynamics of Escherichia coli（EC）,Staphylococcus aureus（SA） and Shigella dysenteriae（SD） on Luria-Bertani（LB） medium under the sterile conditions of 37 ℃ with an inoculum concentration of 1×10~5cell/mL.This method was used to plot growth thremogram curves of these three bacteria upon the treatments by aqueous extracts,alkaloids and nonalkaloid constituents of Coptidis Rhizoma.The differences in the growth metabolism of these bacteria upon various treatments were assayed on the basis of corresponding biothermodynamics parameters（mainly including growth rate constant（k）,growth inhibition ratio（I）,inhibition ratio in half of drug concentration（IC_（50））,maximum heat output（P_（max）） and the time to reach P_（max）（t_（max））.Result：The aqueous extracts and alkaloids significantly inhibited the growth of the three bacteria,and all the orders were alkaloids aqueous extracts;non-alkaloid constituents had no significant inhibitory effects,and even had promotion effects to these three bacteria.The order of the antibacterial ability was SA SD EC for the same extraction part.Conclusion：In summary,the microcalorimetry analysis reveals that in Coptidis Rhizoma,the main active components are alkaloids that display the strongest antibacterial activities against SA.