眼树莲的化学成分分析

Chemical Constituents of Dischidia chinensis

目的:对萝藦科眼树莲Dischidia chinensis全株的化学成分进行分离纯化并对分离得到的单体化合物进行结构鉴定。方法:眼树莲全株干重5.0 kg,粉碎,用95%乙醇室温浸提3次,过滤,合并滤液后经真空减压浓缩得粗浸膏,将其分散于水中成悬浊液,依次用石油醚、乙酸乙酯和正丁醇萃取。运用小孔树脂(MCI),硅胶柱色谱,LH-20羟丙基葡聚糖凝胶(Sephadex LH-20)等柱色谱方法对眼树莲乙酸乙酯萃取物经行分离纯化,应用TLC跟踪监测,分离得到纯度较高的单体化合物。依据各个化合物的理化常数和波谱数据分析对各单体化合物的化学结构进行进一步鉴定。结果:从眼树莲中分离鉴定了10个化合物,分别为β-香树素(1),羽扇豆醇(2),β-香树素乙酸酯(3),3-表木栓醇(4),香草醛(5),4-羟基-3,5-二甲氧基苯甲醛(6),2-羟基-1-(4-羟基-3-甲氧基-苯基)-丙基-1-酮(7),1-(4-hydroxy-3-methoxyphenyl)-1-methoxypropan-2-ol(8),pavonisol(9),S(+)-dehydrovomifoliol(10)。结论:化合物1~10均为首次从眼树莲中分离得到。

Objective：To study the chemical constituents from Dischidia chinensis.Method：The airdried powder of the whole plant of D.chinensis（5kg） was extracted with 95% ethyl alcoholthree times at room temperature to give crude extract.Compounds were isolated and purified by using MCI gel,silica gel,Sephadex LH-20 and their structures were elucidated by physicochemical and organic spetra.Result：Ten compounds were obtained and identified as β-amyrin（1）,lupeol（2）,β-amyrin acetate（3）,3-epifriedelinol（4）,vanillin（5）,4-hydroxy-3,5-dimethoxybenzaldehyde（6）,2-hydroxy-1-（4-hydroxy-3-methoxyp-henyl） propan-1-one（7）,1-（4-hy droxy-3-methoxyphenyl）-1-methoxypropan-2-ol（8）,pavonisol（9）,and S（＋）-dehydrovomifoliol（10）,respectively.Conclusion：The compounds 1-10 were obtained from the D.chinensis for the first time.