基于护场理论探讨红肿消酊箍围法对皮肤脓肿大鼠创周组织IL-10,IL-1β基因表达的影响

Effect of Hongzhongxiao Tincture “Hoop Circumference” Method on Gene Expression of IL-10 and IL-1β in Tissues Around Wound of Rats with Skin Abscess Under Guidance of Theory of “Defended Field”

目的:观察护场理论指导下红肿消酊剂箍围法对皮肤脓肿大鼠创周组织白细胞介素-10(IL-10),白细胞介素-1β(IL-1β)基因表达水平的影响。方法:32只大鼠分为空白组、模型组、莫匹罗星软膏组及红肿消酊剂组。除空白组外,其余3组皮下注射金黄色葡萄球菌1 mL(3×10~9~5×10~9cfu·mL^(-1))建立皮下脓肿模型。模型组生理盐水换药,莫匹罗星软膏组采用莫匹罗星软膏2 g外涂换药,红肿消酊剂组采用红肿消2 mL换药,用药范围超过肿胀范围1 cm,换药1次/d。分别于治疗3,7,14,18 d取距离大鼠创面创周1,2,3 cm组织,观察大鼠创面脓肿局限及愈合时间,并检测IL-10,IL-1βmRNA转录水平。结果:与模型组比较,红肿消酊剂组能够缩短脓肿局限时间及创面愈合时间(P<0.05,P<0.01),红肿消酊剂组与莫匹罗星软膏组比较无统计学差异。红肿消酊剂组距离创周不同范围组织中IL-10水平在治疗过程中呈上升趋势,高于莫匹罗星软膏组及模型组,红肿消酊剂组治疗早期IL-1β水平低于莫匹罗星软膏组及模型组(P<0.05,P<0.01)。结论:红肿消酊剂箍围能够提高IL-10水平,并在脓肿形成早期抑制组织内IL-1β表达,从而能够有效减轻脓肿局部炎症反应,促进脓肿局限及创面愈合,为护场形成提供条件。

Objective：To observe the effect of ＇hoop circumference＇（Hongzhongxiao tincture） on the gene expression levels of interleukin-10（IL-10） and interleukin-1β（IL-1β） in the tissues around the wound of rats with skin abscess under the guidance of the theory of ＇defended field＇.Method：The 32 rats were divided into blank group,model group,Mupirocin ointment group and Hongzhongxiao tincture group.Except the rats in blank group,all the rats in other three groups received subcutaneous injection of Staphylococcus aureus 1 mL（3×10~9-5×10~9cfu·mL^（-1）） to establish subcutaneous abscess models.The rats in model group received normal saline for treatment.Mupirocin ointment 2 g dressing was given in Mupirocin ointment group;and 2 mL Hongzhongxiao dressing was given in Hongzhongxiao tincture group,1 cm over the swelling range,1 time a day.The tissues 1,2,3 cm around the wound were taken on day 3,7,14 and 18 of treatment to observe the healing time and wound abscess limitation time;in addition,the transcription levels of IL-10 and L-1β mRNA were detected.Result：As compared with model group,Hongzhongxiao tincture group could shorten the time of the abscess and wound healing time,（P〈0.05,P〈0.01）;however,there was no significant difference between Hongzhongxiao tincture group and Mupirocin ointment group.The IL-10 level was increased in Hongzhongxiao tincture group,and higher than that in the model group and Mupirocin ointment group during the treatment;while IL-1β level in Hongzhongxiao tincture group was lower than that in the Mupirocin ointment group and model group at the early treatment stage（P〈0.05,P〈0.01）.Conclusion：The Hongzhongxiao tincture can increase IL-10 level,and inhibit the expression of IL-1β in the tissues in the early stage of abscess formation,thus reducing the local inflammatory reaction of the abscess effectively,promoting the limitation of abscess and wound healing,and providing conditions for the formation of the ＂defended field＂.