基于网络药理学的吴茱萸汤作用机制分析

Study on Mechanism of Wuzhuyu Tang Based on Network Pharmacology

目的:探讨吴茱萸汤的功效物质基础和配伍机制。方法:依托中药系统药理学分析平台(TCMSP)检索吴茱萸汤全方4味中药的化学成分和作用靶点。进而构建化合物-靶点网络、蛋白互作(PPI)网络、靶点-通路网络,研究吴茱萸汤作用机制。结果:化合物-靶点网络包含29个化合物和相应靶点77个,关键靶点涉及PTGS2,PTGS1等。PPI网络包含70个蛋白,关键蛋白涉及AP-1,BCL2,HSP90α等。基因本体(GO)条目145个,其中生物过程相关的条目100个,分子功能相关的条目23个,细胞组成相关的条目22个。京都基因与基因组百科全书(KEGG)通路3条,涉及钙离子信号通路、神经活性配体-受体相互作用信号通路、癌症信号通路。结论:本研究结果初步验证了吴茱萸汤的基本药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础。

Objective：To investigate the efficacy and compatibility mechanism of Wuzhuyu Tang.Method：The chemical components and targets related to the four Chinese herbal medicines were searched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（TCMSP）.The compoundtarget network,protein-protein interaction（PPI） network,and target-pathway network were constructed to explore the mechanism of Wuzhuyu Tang.Result：The compound-target network contain 29 chemicalcomponents and 77 targets.The hub targets involve PTGS2,PTGS1 and so on.The PPI network contain 70 proteins,in which the hub proteins involve AP-1,BCL2,HSP90α and so on.In the gene ontology（GO） functional enrichment analysis,there are 145 GO terms（100 Biological Process,23 Molecular Function,22 Cellar Component）.The pathway enrichment analysis contains 3 pathways：calcium signaling pathway,neuroactive ligand-receptor interaction,and pathways in cancer.Conclusion：The result of the study preliminarily verifies the basic pharmacological effects and related mechanisms of Wuzhuyu Tang,thuslaying a solid foundation for deeply study on the mechanism of action.