胃癌组织中IL-22BP、IL-22、STAT3的表达变化及意义

Expression and clinical significance of IL-22BP,IL-22,and STAT3 in human gastric cancer tissues

目的观察白细胞介素22结合蛋白(IL-22BP)、IL-22、信号传导和转录激活因子3(STAT3)在胃癌组织中的表达变化,并探讨其意义。方法采用免疫组化SP法对经手术后的37例份胃癌组织及其配对的距癌旁5 cm外的正常胃组织标本37例份进行染色,检测IL-22BP、IL-22、STAT3表达,并分析三者与胃癌患者临床病理特征的关系。结果在胃癌组织、正常胃组织中IL-22BP阳性表达率分别为67.6%(25/37)、18.9%(7/37),IL-22阳性表达率分别为73.0%(27/37)、18.9%(7/37),STAT3阳性表达率分别为75.8%(28/37)、24.3%(9/37),胃癌组织、正常胃组织中IL-22BP、IL-22、STAT3表达差异均有统计学意义(P均<0.01)。IL-22BP、IL-22、STAT3在胃癌组织中的阳性表达与患者临床分期、浸润深度、淋巴结转移均有关(P均<0.05),与患者的性别和年龄无关(P均>0.05);胃癌组织中IL-22BP表达与IL-22、STAT3表达呈正相关(r分别为0.526、0.383,P均<0.05);IL-22表达与STAT3表达呈正相关(r=0.342,P<0.05)。结论胃癌组织中IL-22BP、IL-22、STAT3呈高表达,且三者表达与胃癌的侵袭和转移有关,三者可能协同促进胃癌的发生发展。

Objective To detect the expression of interleukin-22（ IL-22） binding protein（ IL-22BP）,IL-22,signal transducer and activator of transcription-3（ STAT3） in the tissues of patients with gastric cancer,and to explore its significance. Methods Using immunohistochemical SP method to stain 37 cases of gastric cancer tissues and their matched normal gastric tissues which were obtained from the proximal margin of the resection and beyond 5 cm to the gastric cancer section,the expression of IL-22 BP,IL-22,and STAT3 was detected,and the relationships of the three parameters with clinicopathological features of gastric cancer were analyzed. Results The positive expression rates of IL-22 BP in the gastric cancer tissues and the normal gastric tissues were 67. 6%（ 25/37） and 18. 9%（ 7/37）; the positive expression rates of IL-22 in the gastric cancer tissues and the normal gastric tissues were 73. 0%（ 27/37） and 18. 9%（ 7/37）; the positive expression rates of STAT3 in the gastric cancer tissues and the normal gastric tissues were 75. 8%（ 28/37） and 24. 3%（ 9/37）,respectively. Significant differences were found in the positive expression rates of the three parameters between the gastric cancer tissues and the normal gastric tissues（ all P 〈 0. 01）. The positive expression of IL-22 BP,IL-22,and STAT3 in the gastric cancer tissues was significantly related with the clinical stage,depth of invasion,and lymph node metastasis of the patients with gastric cancer（ all P 〈 0. 05）,but not related with gender and age（ all P 〉 0. 05）. In the gastric cancer tissues,the expression of IL-22 BP was positively correlated with the expression of IL-22,and STAT3（ r =0. 526,0. 383,respectively; all P 〈 0. 05）,and the expression of IL-22 was positively correlated with the expression of STAT3（ r = 0. 342,P 〈 0. 05）. Conclusion IL-22 BP,IL-22,and STAT3 are highly expressed in the gastric cancer tissues,the expression of them is related with the invasion and metastasis of gastric cancer,and the results suggest that they may synergistically promote the occurrence and development of gastric cancer.