摘要
[Objective ] The paper was to investigate the major cold-resistant materials and their content variation in the diapause process of Exorista civilis Ronda- hi. [Method] The total carbohydrate, protein and glycerin of E. civilis Randani in the diapause process were examined. [Result] The glycogen content in dia- pause stage was higher than that in non-diapause stage, and the difference was significant; the glycogen contents in pupal stage reached the maximum values of 156. 73 and 68.32 p~/mg, respectively. The protein content was higher in non-diapause stage of E. civilis Rondani before entering diapause stage, and reached the max- imum value of 78.34 μg/mg in the pupal stage ; while the maximum value in non-diapanse stage was 72.95μg/mg. The glycerin content of adults in non-diapause and diapanse stage was very low; the glycerin concentration in diapause stage was higher than that in non-diapause stage throughout the whole development stage ex- cept the pupal stage, and the difference was significant. [ Conclusion] Glycogen is the most important cold resistant substance in the diapause process of E. civilis Rondani.
[Objective ] The paper was to investigate the major cold-resistant materials and their content variation in the diapause process of Exorista civilis Ronda- hi. [Method] The total carbohydrate, protein and glycerin of E. civilis Randani in the diapause process were examined. [Result] The glycogen content in dia- pause stage was higher than that in non-diapause stage, and the difference was significant; the glycogen contents in pupal stage reached the maximum values of 156. 73 and 68.32 p~/mg, respectively. The protein content was higher in non-diapause stage of E. civilis Rondani before entering diapause stage, and reached the max- imum value of 78.34 μg/mg in the pupal stage ; while the maximum value in non-diapanse stage was 72.95μg/mg. The glycerin content of adults in non-diapause and diapanse stage was very low; the glycerin concentration in diapause stage was higher than that in non-diapause stage throughout the whole development stage ex- cept the pupal stage, and the difference was significant. [ Conclusion] Glycogen is the most important cold resistant substance in the diapause process of E. civilis Rondani.
基金
Supported by National Key R&D Project“Prevention and Control Techniques and Product Development of Natural Enemies”(SQ2017ZY060059)
