拨法对CCI模型大鼠外周血清中IL-1β以及脊髓中5-HT_(2A)表达的影响

Effect of Bo'fa method on the expression of IL-1β in peripheral serum and 5-HT_(2A) in spinal cord of CCI model rats

目的从行为学和形态学角度探索拨法对CCI模型大鼠感觉功能恢复的影响。方法采用推拿手法模拟仪定性、定量模拟手法,对CCI模型大鼠进行干预。通过光热耐痛阈分析大鼠行为学改善情况,ELISA法测定血清中IL-1β含量的变化,Western blot法检测脊髓中5-HT_(2A)受体的相对蛋白表达情况。结果拨法治疗组造模后7天的光热耐痛阈数值与模型组无差异(P>0.05),经过拨法治疗20次后(即造模后28天),二者之间有显著差异(P<0.05),且拨法组逐渐接近正常组水平。ELISA法检测血清中IL-1β的含量:造模后7天,模型组和拨法组IL-1β含量均显著高于正常组,经过拨法治疗20次后,拨法组IL-1β与模型组相比显著性下降(P<0.05)。Western blot法测定脊髓中5-HT_(2A)的相对蛋白表达含量:拨法治疗20次后,拨法组脊髓中5-HT_(2A)相对蛋白表达含量明显上调,且已接近正常组水平(P>0.05),高于模型组水平(P<0.05)。结论经过拨法治疗,可明显上调CCI模型大鼠的光热耐痛阈值,减轻其痛觉过敏状态;下调CCI模型大鼠外周血清中促炎性细胞因子IL-1β的表达,达到"消炎镇痛"的作用;上调脊髓中5-HT_(2A)受体的表达,对中枢镇痛起到一定作用。

Objective Explore the effects of Bo＇fa method on the recovery of sensory function of the CCI model rats from the behavior and morphology. Methods The CCI model rats were intervened qualitatively and quantitatively by the massage simulation instrument. The variation of rats＇ behavior was analyzed through the solar-thermal pain threshold. The changes of the IL-1β in the serum were measured by ELISA. And we detected the relative protein expression of 5-HT2A receptor in the spinal cord by Western blot. Results There was no significant difference in the results of solar-thermal pain threshold between the Bo＇fa method group and the model group at the 7^（th） day after modeling（ P〈0.05）. After twenty times of Bo＇fa method treatment,the difference between the two groups became statistically significant（ P〈0.05）,and the results were at or near the normal group level. At the 7^（th） day after modeling,the levels of IL-1β in serum measured by ELISA in the model group and the Bo＇fa method group were significantly higherthan those in the normal group. After 20 times of Bo＇fa method treatment,the levels of IL-1β in the Bo＇fa method group declined significantly compared with those in the model group（ P〈0.05）. The relative protein expression of 5-HT2A in the spinal cord was measured by the method of Western blot. The results of this experiment indicated that,after 20 times of Bo＇fa method treatment,the relative protein expression of 5-HT2A in the spinal cord in the Bo＇fa method group increased significantly higher than that in the model group（ P〈0.05）,and became close to that in the normal group（ P〈0.05）. Conclusion Bo＇fa method treatment can increase obviously the values of solar-thermal pain threshold of CCI model rats,ease the recovery of sensory function,decrease the expression of pro-inflammatory cytokine IL-1β in peripheral serum of the CCI model rats to eliminate the inflammation of NPP. Moreover,Bo＇fa method can increase the expression of 5-HT2A receptor in the spinal cord,playing an important role in the analgesia of central nerves.