摘要
采用醇沉水提法从资源植物玉叶金花果实中提取粗多糖,通过乙醚脱脂、Savage法脱蛋白得到精多糖。在单因素试验的基础上,采用L9(34)正交试验,探讨pH值、浸提时间、料液比、乙醇醇析体积倍数对玉叶金花果实中精多糖提取率的影响。结果表明,玉叶金花果实中精多糖的最佳提取工艺条件为料液比1∶15(g·m L-1),浸提时间3 h,pH值6,乙醇醇析体积倍数1∶4。在此提取工艺条件下,提取率为2.71%;通过验证此最佳提取工艺稳定可行。
The crude polysaccharide in the resource plant of Mussaenda esquirolli L6vl. fruit was extracted by alcohol-water extrac- tion, and then the refined polysaccharide were got using degreasing of ether and eluting protein of Sevage reagent. Comparison of sin- gle-factor test and then using orthogonal test of L9 （3^4）, 4 factors such as pH, extraction time, liquid ratio, times the size of ethanol al- cohol -precipitated to the refined polysaceharide extraction were studied with the extract yield for the evaluation. The results showed that the best conditions for extraction were extracting liquid than 1：15 （g. mL^-1）, extraction time 3 h, pH 6, 4 times the size of ethanol alcohol-precipitated. Under this extraction process conditions, extract yield was 2.71%, the optimum extraction process was stable and feasible.
出处
《天津农业科学》
CAS
2017年第8期15-19,共5页
Tianjin Agricultural Sciences
基金
安徽省大学生创新训练项目(201510375011)
安徽省重大教学研究项目(2016jyxm0981)
安徽省校企合作实践教育基地项目(2015sjjd022)
关键词
资源植物
玉叶金花
多糖
单因素试验
正交试验
resource plant
Mussaenda esquirolli Levl.
polysaccharide
single-factor test
orthogonal test
