共培养下前列腺增生上皮细胞及尿液对尿路上皮细胞增殖的影响

Effects of benign prostatic hyperplasia cells and urine on the proliferation of urinary tract epithelial cells

目的研究前列腺增生细胞及尿液对尿路上皮细胞增殖的影响,进一步探讨前列腺部尿道术后的修复机制。方法采集10例需行经尿道前列腺电切术患者术前1d,术后6h、术后2d及术后5d尿液标本,通过ELISA法测定标本中TGF-β_1含量;分别使用不同浓度人天然尿液刺激HUC细胞24h后,通过CCK8检测HUC细胞增殖情况;使用Transwell实验检测BPH细胞及尿液对HUC细胞的增殖影响。结果前列腺电切术后患者第2天尿液中TGF-β_1含量较术前有所增高[(1.208±0.018)ng/L],且具有统计学意义(P<0.000 1),尿液对于HUC促增殖作用在尿液稀释浓度为1∶2及1∶4具有统计学意义(A值分别为1.138±0.027和1.171±0.029,P<0.05),其他浓度则无统计学意义,且最适刺激浓度为1∶4;共培养组及含尿液共培养组细胞增殖都比对照组明显,且含尿液共培养组比单纯共培养组增殖的更加明显(A值分别为0.499±0.005和0.516±0.005,P<0.05)。结论患者术后尿液中TGF-β_1含量较术前有所增高,尿液及BPH细胞对于HUC细胞具有促增殖作用,并且两者同时影响HUC细胞时促增殖现象更加明显。

Objective To research the effect of benign prostatic hyperplasia（BPH） cells and urine on the proliferation of human urinary tract epithelial cells（HUCs）,and to investigate the repair mechanism of prostatic urethra after transurethral resection of the prostate.MethodsUrine samples were collected from 10 patients who underwent transurethral resection of the prostate（TURP） at different time points（1 day before operation,and 6 hours,2 days and 5 days after operation）.The expre-ssion of TGF-β1 was tested with ELISA.After the HUCs were stimulated with different concentrations of human urine for 24 hours,the proliferation was detected with CCK8; the effects of BPH cells and urine on the proliferation were determined with Transwell assay.ResultsThe Leve of TGF-β1 rose in the 2-day sample[（1.208±0.018）ng/L,P〈0.000 1].The effects of urine on the proliferation of HUCs in the urine dilution concentration of 1∶ 2 and 1∶ 4 were statistically significant[A=（1.138±0.027） and （1.171±0.029）,P〈0.05],while other concentrations had no effects.The optimal stimulation concentration was 1∶4,and the cell proliferation of co-culture group and urine co-culture group were significantly higher than that of the control group [A=（0.499±0.005） and （0.516±0.005）,P〈0.05],and the proliferation of the urine co-culture group was more obvious than that of the co-culture group.ConclusionThe expression of TGF-β1 in urine from TURP patients increases 2 days after surgery.Both BPH cells and urine can promote the proliferation of HUCs,and have greater effect when they affect the HUCs at the same time.