水通道蛋白11在羊水量过少产妇胎盘和胎膜中的表达变化及意义

Expression and significance of aquaporin 11 in placenta and fetal membranes of term parturient with oligohydramnios

目的探讨水通道蛋白(AQP)11在特发性羊水过少产妇胎盘和胎膜中定性及相对定量表达情况及其在羊水平衡过程中的作用。方法选择在兰州大学第一医院经剖宫产分娩的55例产妇,其中25例特发性羊水过少者作为实验组,30例羊水量正常的足月产妇为对照组。采用SABC免疫组化法和实时荧光定量PCR技术,检测两组产妇羊膜、绒毛膜、胎盘中AQP11的表达及分布。结果 AQP11在足月妊娠产妇羊膜、绒毛膜及胎盘中均有表达,且在羊膜的表达与羊水量呈正相关,在胎盘的表达与羊水量呈负相关;在不同羊水量的绒毛膜的表达均呈增加趋势。结论AQP11在妊娠晚期羊水量调节中可能发挥着重要作用。

Objective To explore the expression and localization of aquaporin 11 （AQPll） in human term pregnancies with oligihydramnios, and its role in amniotic fluid balance. Methods We studied 55 patients who underwent elective cesarean sections, consisting of 25 patients with isolated oligohydramnios and 30 with normal amniotic fluid volume. Immunohistochemistry and reverse transcription polymerase chain reaction were employed to determine the expression and localization of AQP11 in the amnion, chorion and placenta. Results AQP11 protein was detected in expressed in the full-term pregnant women＇s amnion, chorion and placenta. The expression in the amnion was positively correlated with amniotic fluid amount; the expression in the placenta was negatively correlated with amniotic fluid amount. The expression increased in the chorion with different amniotic fluid amount. Conclusion AQP11 plays an important role in regulating amniotic fluid balance.