楠竹叶提取物对D-半乳糖诱导的小鼠认知损伤和氧化应激损伤的改善作用

Bamboo Leaf Extracts of Phyllostachys pubescens Cognitive Impairment and Attenuate Oxidative Damage in the D-Galactose-Induced Aging Mouse

目的:研究楠竹的竹叶提取物(bamboo leaf extracts,BLE)对D-半乳糖(Dgalactose,D-gal)诱导的小鼠认知损伤和氧化应激损伤的改善作用。方法:32只小鼠随机分为对照组、D-gal组、BLE组(1 g·kg^(-1))和维生素E(VE)组(100 mg·kg^(-1))。D-gal组、BLE组和VE组采用皮下注射D-gal 50 mg·kg^(-1),每天一次,连续给药共8周造模。水迷宫实验检测小鼠学习记忆能力,同时测定海马中超氧化合物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、谷胱甘肽(GSH)的含量。免疫印迹法检测海马中突触后密度蛋白-95(PSD-95)和突触小泡蛋白(SYN)表达情况。结果:给予BLE饲料后,D-gal诱导的小鼠学习记忆损伤得到改善。脑组织中SOD、CAT和GSH的含量增加,MDA的含量减少。D-gal可降低海马中PSD-95和SYN的蛋白表达,而给予BLE饲料后,海马中PSD-95和SYN的蛋白表达增加。结论:BLE可改善D-gal诱导的小鼠学习记忆障碍和脑损伤。

Objective： To identify whether supplementation with bamboo leaf extracts （BLE） of Phyl- lostachys pubescens in diet has a protective effect against D-galactose （D-gal）-induced cognitive impairment and oxidative damage in mice. Methods： Thirty-two mouse were randomly divided into four groups, includ- ing control group, D-gal group, BLE （1 g.kg-1） group and vitamin E （VE, 100 mg＇kg-1） group. Each group was given ip D-galactose 50 mg＇kg-1 per day except the control group was supplied with equal amount of physiological saline for 8 weeks. The Morris water maze test was used to detect the ability of learning and memory in mice. Meanwhile, the activities of superoxide dismutase （SOD） and catalase （CAT）, the contents of glutathione （GSI-I） and malondialdehyde （MDA） in the hippocampus tissues were determined. And the ex- pressions of PSD-95 and SYN in the hippocampus were assessed by Western blots. Results： Compared with the model of G-gal-treated mice, BLE improved learning and memory abilities. And BLE reduced the content of MDA, increased the activities of SOD and the content of GSH in the hippocampus tissues. Conclusion： These results indicated that BLE could in D-gal-treated mice. improve cognitive functions and attenuate brain injury