消癥软肝方联合5-氟尿嘧啶对H_(22)荷瘤小鼠相关指标的影响

Effect of Xiaozheng Ruangan Formula Combined with 5-Fluorouracil on Relevant Indexes of H_(22) Lumor-Bearing Mice

目的:探讨中医药在肝癌的中西医结合治疗中所发挥的作用。方法:通过癌株传代法建立H_(22)肝癌小鼠模型,将造模成功的40只肝癌小鼠随机分为模型组、消癥软肝方组、5-氟尿嘧啶组以及消癥软肝方联合5-氟尿嘧啶组。中药组以消癥软肝方配方颗粒0.2 mL/d剂量灌胃治疗,西药组以5-氟尿嘧啶20 mg/kg·d^(-1)腹腔注射治疗,联合用药组给予中西药物联合治疗,连续给药12 d。结果:联合用药组小鼠的脾指数、胸腺指数明显好于西药组,抑瘤率和血管内皮生长因子(VEGF)、基质金属蛋白酶9(MMP-9)蛋白的表达都明显优于中药组和西药组。结论:消癥软肝方具有明显的抗癌作用,能够增强5-氟尿嘧啶的抗癌效果,其作用机制与抑制肿瘤血管生成有关。

Objective： to explore the effect of Chinese medicine in treating hepatocarcinoma with combined therapy of TCM and western medicine.Methods： 40 H22 hepatocarcinoma model mice, made by cancer strain passage method, were randomly divided into model group, Chinese medicine group （administrated with Xiaozheng Ruangan formula granules by 0.2 mL/d）, western medicine group （injected with 5-fluorouracil by 20 mg/kg·d-1）, and combination group.The administration lasted for 12 days.Results： spleen index and thymus gland index of combination group were obviously higher than that of western medicine group;inhibition rate, vascular endothelial growth factor （VEGF） and matrix metalloproteinase-9 （MMP-9） expressions were superior to that of Chinese and western medicine groups.Conclusion：Xiaozheng Ruangan Formula has obvious anticancer effect and can enhance the antitumor effect of 5-fluorouracil, and its mechanism is related to the inhibition of tumor angiogenesis.