丹参通络解毒汤对骨髓干细胞移植急性心肌梗死模型大鼠NF-κB介导的炎症反应影响

Effects of Danshen Tongluo Jiedu Decoction on NF-kappa B Mediated Inflammatory Response in Bone Marrow Stem Cell Transplantation Rats With Acute Myocardial Infarction

目的:探讨丹参通络解毒汤对骨髓干细胞(BMSCs)移植急性心肌梗死(AMI)模型大鼠核因子-κB(NF-κB)介导炎症反应的影响。方法:80只大鼠按随机数字表法分为SH组(假手术组)、AMI组(模型组)、BMSCs组(BMSCs移植组)、DSTLJD组(丹参通络解毒汤组)、DSTLJD+BMSCs组(丹参通络解毒汤+BMSCs移植)各10只,BMSCs细胞悬液直接注入梗死区边缘心肌组织,给予中药或生理盐水灌胃,4周后采用酶联免疫吸附法(ELISA)测定各组大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的含量,免疫组化法观察心肌核因子-κB(NF-κB)蛋白表达,并用HE染色观察心肌组织形态病理变化。结果:与AMI组比较,BMSCs组、DSTLJD组、DSTLJD+BMSCs组大鼠心肌NF-κB蛋白表达明显降低,且血清炎症因子TNF-α和IL-6水平明显下降,差异有统计学意义,心肌损伤程度明显改善;DSTLJD+BMSCs组优于单纯BMSCs组、单纯DSTLJD组,差异有统计学意义。结论:BMSCs移植联合丹参通络解毒汤能够改善AMI模型大鼠的心肌损伤,减轻炎症反应,修复缺血心肌,其机制可能与调节NF-κB介导的炎症反应有关。

Objective： To explore the effect of Danshen Tongluo Jiedu Decoction on the inflammatory reaction mediated by NF-κB in acute myocardial infarction（ AMI） rats by Bone marrow stem cell transplantation. Methods： The whole bone marrow adherent method was adopted for BMSCs separation and culture. The AMI model was established by closing the left anterior descending coronary artery of SD rats. SD rats were randomly divided into SH（ sham group）,AMI group（ acute myocardial infarction group）,BMSCs（ BMSCs transplantation group）,DSTLJD group（ Danshen Tongluo Jiedu group）,DSTLJD ＋ BMSCs group（ Danshen Tongluo Jiedu combined with BMSCs transplantation group）,10 in each group.BMSCs cell suspension injected directly into the edge of the myocardial tissue infarction area; Chinese medicine or physiological saline were administered by gavage. 4 weeks later,by using enzyme-linked immunosorbent（ ELISA） measure each group rats serum TNF – α、IL-6 content was detected. Using immunohistochemical method to observe the myocardial NF-κB protein expression,and HE staining to observe the myocardial tissue pathological changes. Results： Compared with The AMI group,BMSCs,DSTLJD group,DSTLJD ＋ BMSCs group rats serum level of TNF-α、IL-6 and myocardial nuclear factor NF-κB protein expression were significantly reduced,the difference has statistically significant,the degree of myocardial injury was significantly improved; DSTLJD ＋ BMSCs group was better than that of pure stem cell transplantation,and simple DSTLJD group,the difference has statistically significant. Conclusion： Bone marrow stem cell transplantation combined with Danshen Tongluo Jiedu Decoction can improve the myocardial injury, reduced the inflammatory reaction,and repair the ischemic myocardium in AMI rats. The mechanisms may be related to regulate the inflammatory reaction mediated by NF-κB.