金番止泻胶囊对脾虚小鼠肠道菌群的影响及体外抗菌作用

Effect of Jinfan Zhixie Capsule on Gut Flora in Spleen-deficiency Mice and in Vitro Bacteriostasis

目的:探讨金番止泻胶囊对脾虚小鼠肠道菌群的调节作用及体外抗菌作用。方法:利用番泻叶灌胃加饥饱失常复制小鼠脾虚模型,经金番止泻胶囊治疗后,以菌落计数法检测其盲肠内容物中双歧杆菌、乳酸杆菌、肠球菌及肠杆菌的数量;采用纸片扩散法、试管2倍稀释法考察金番止泻胶囊对枯草杆菌、大肠杆菌、金黄色葡萄球菌、痢疾杆菌、伤寒杆菌的抑菌作用。结果:与正常组比较,模型组双歧杆菌、乳酸杆菌、肠球菌及肠杆菌数量均升高,金番止泻胶囊高(1.2 g·kg-1)、低剂量组(0.6 g·kg-1)指标均有一定的恢复并接近正常水平。对枯草杆菌、大肠杆菌、金黄色葡萄球菌、痢疾杆菌、伤寒杆菌的MIC(mg·m L-1)分别为12.8、8.51、6.4、7.6、8.27。结论:金番止泻胶囊对脾虚小鼠肠道菌群有调节作用,在体外有抑菌作用。

Objective： To observe the effects of gut flora in spleen-deficiency mice and in vitro bacteriostasis of Jinfan Zhixie Capsule（ JZC）. Methods： Establishing the spleen-deficiency mice model with senna extract and fully and poorly feeding food in turn,and then detect the number of Bifidobacterium,Lactobacillus,Enterococcus and Escherichia coli in mice cecum contents by colo-ny counting after treated with JZC. By filtering paper method and tube double dilution method to inspec the bacteriostasis effect for Bacillus subtilis,Escherichia coli,Staphylococcus aureus,Shigella,Salmonellatyphi on JZC. Results： Compared with the normal control group,the number of Bifidobacterium,Lactobacillus,Enterococcus,and Escherichia coli in mice cecum contents increased in model group. After treated with the 1. 2 g·kg-1and 0. 6 g·kg^（-1） of JZC,all the indexes have certain recovery and close to the normal level. The MIC values（ mg · mL^（-1）） againsts Bacillus subtilis,Escherichiacoli,Staphylococcus aureus,Shigella,Salmonellatyphi respectively were 12. 8、8. 51、6. 4、7. 6、8. 27.Conclusion JZC has dependent manner recovered the gut flora in spleen-deficiency mice,and in vitro bacteriostasis.