不同剂量川芎-当归、三棱-莪术对AS小鼠Cyr61、Sgk1、Ch25h基因表达水平的影响

Effects of Different Doses of Ligusticum-Chuanxiong,Angelica Sinensis and Rhizoma Curcumae on the Expression of Cyr61,Sgk1 and Ch25h Genes in As Mice

目的:探讨不同剂量川芎-当归、三棱-莪术对载脂蛋白E(Apoe)基因缺陷小鼠动脉粥样硬化(AS)模型主动脉组织Cyr61、Sgk1、Ch25h基因表达水平的影响。方法:将64只5周龄Apoe基因缺陷小鼠按随机数字表法分为模型对照组、阿托伐他汀钙组、低活血药组、高活血药组、低破血药组、高破血药组6组,造模成功后分别灌胃给药连续8周,处理动物并用基因芯片技术检测小鼠主动脉组织中差异表达基因,并通过RT-PCR法进行验证。结果:川芎-当归、三棱-莪术均能下调Cyr61、Sgk1、Ch25h基因表达水平且存在量效关系。结论:活血药(当归、川芎)、破血药(三棱、莪术)具有抗动脉粥样硬化的作用,其作用机制可能与调节主动脉Cyr61、Sgk1、Ch25h基因表达水平有关。

Objective： To observe the effect of different dosages of blood-activating medicines（ Angelica sinensis and ligusticum wallichii） and blood-breaking medicines（ Rhizoma sparganii and Curcuma zedoary） on Cyr61、Sgk1 and Ch25 h Gene Expression in thoracic aorta of Apo E Gene Deficient Mice atherosclerosis（ AS） Models. Methods： 64 five-week-old Apo E-gene deficient mice were randomly divided into 6 groups： the model group,the atorvastatin group（ statin group）,low dose of blood-activating group（ low blood-activating group）,low dose of blood-breaking group（ low blood-breaking group）,high dose of blood-activating group（ high blood-activating group）,high dose of blood-breaking group（ high bloodbreaking group）. The AS modes of mice were built with basic food which were killed after 8-week drug gastric perfusion.The differential gene expression of thoracic aortas of mice were analysed by gene test. Then these genes in mice were detected by realtime PCR. Results： Both blood-activating medicines and blood-breaking medicines could cut the Cyr61、Sgk1 and Ch25 h gene expression levels,and there was a significant dose effect relationship between the high and low dose groups. Conclusion： Blood-activating and blood-breaking medicines show anti-atherosclerotic effect and their mechanism may be related with Cyr61、Sgk1 and Ch25 h gene expression level ajustment in aorta.