甲胎蛋白阴性与阳性穿刺活检人肝细胞癌中CD34及多克隆癌胚抗原的表达及其病理诊断意义

Expression of CD34 and polyclone carcinoembryonic antigen detected by puncture biopsy of positive and negative alpha fetoprotein in human hepatocellular carcinoma tissue and its significance of pathological diagnosis

目的检测甲胎蛋白(alpha fetoprotein,AFP)阴性与AFP阳性穿刺活检人肝细胞癌(hepatocellular carcinoma,HCC)中CD34及多克隆癌胚抗原(polyclone carcinoembryonic antigen,p CEA)的表达情况,探讨其病理诊断意义。方法收集2013年—2015年恩施土家族苗族自治州中心医院穿刺活检病理确诊为HCC标本54例,依据血清学及免疫组织化学法(免疫组化)检测AFP结果,将患者分为AFP阳性组(n=33)及AFP阴性组(n=21)。应用免疫组化技术,检测54例HCC组织中CD34、p CEA的表达情况。结果 AFP阳性组p CEA的阳性表达率(69.7%)高于AFP阴性组(38.1%),差异有统计学意义(P<0.05);而CD34在AFP阳性组(90.91%)与AFP阴性组(85.71%)中的阳性表达率比较,差异无统计学意义(P>0.05)。结论 AFP与CD34、p CEA联合检测有利于肝穿刺活检中HCC的诊断及其鉴别诊断。

Objective To explore the expression of CD34 and polyclone carcinoembryonic antigen （pCEA） of positive and negative alpha fetoprotein （AFP） detected by puncture biopsy in human hepatocellular carcinoma （HCC） and the significance of pathological diagnosis. Methods Fifty-four HCC tissue specimens from 2013 to 2015 were collected from tumor biopsy samples which confirmed by pathology in the Central Hospital ofEnshi Tujia and Miao Autonomous Prefecture. The tissue samples were divided into positive AFP group （n=33） and negative AFP group （n=21） according to the detection results of serology and immunohistochemistry analysis of AFP. Expressions of CD34 and pCEA in the fifty- four HCC specimens were detected by immunohistochemistry. Results The positvie expression rate of pCEA in the positive AFP group was 69.7%, which was significantly higher than that in the negative AFP group （38.1%） （P〈0.05）. However, the difference in positive expression rate of CD34 between the positive and negative AFP groups （90.91% and 85.71%, respectively） was not significant （P〉O.05）. Conclusion The associated detection ofAFP, pCEA and CD34 in HCC tissues might contribute to the pathological and differential diagnosis of human hepatocellular carcinoma in puncture biopsies.