外周血T细胞亚群及NK细胞比例在转移性结肠癌患者预后评估中的临床意义

The role and mechanism of autophgy in hepatocellular carcinoma

目的探讨反复化疗的转移性结肠癌患者(mCRC)T淋巴细胞亚群和自然杀伤(NK)细胞比例及其临床意义。方法将我院30例转移性结肠癌患者作为观察组,回顾性分析外周血T淋巴细胞亚群及NK细胞的检测结果,与30例健康受检者(对照组)进行比较分析,并于化疗4周期后复查结肠癌患者的外周血T淋巴细胞亚群及NK细胞,对比有效与无效患者的检测结果。结果 mCRC患者中化疗有效组化疗后外周血中B细胞、CD4^+细胞、NK细胞及CD4^+/CD8^+比值均明显高于同组化疗前水平(11.2%±0.98%vs 6.7%±1.05%,P<0.05;30%±2.01%vs 26%±2.02,P<0.05;10%±1.02%vs8.9%±0.72%,P<0.05;1.2±0.03 vs0.9±0.06,P<0.05),而化疗无效组化疗前后差异均无统计学意义(P>0.05)。化疗无效组外周血中B细胞、CD4^+细胞、NK细胞及CD4^+/CD8^+比值均明显低于正常对照组(2.4%±0.01%vs15%±1.10%,P<0.05;8%±0.03%vs37%±2.10%,P<0.05;4.4%±0.12%vs25%±1.80%,P<0.05;0.44±0.01 vs1.23±0.02,P<0.05)。结论 NK细胞与外周血T淋巴细胞亚群对评价结肠癌化疗的疗效、判断预后具有重要价值。

Objective To study the chemotherapy of patients with metastatic colorectal cancer（mCRC） repeatedly T lymphocyte subgroup and natural killer（NK）cell proportion and its clinical significance. Methods 30 patients with metastatic colon cancer patients as observation group,a retrospective analysis of peripheral blood T lymphocyte subsets and NK cells test results,and 30 cases of healthy subjects（control group）comparative analysis,and review the situation after 4 cycles of chemotherapy in patients with colon cancer in the peripheral blood T lymphocyte subsets and NK cells,compared to patients with valid and invalid test results.Results The mCRC effective chemotherapy group after chemotherapy in patients with B cell in peripheral blood,CD4^＋cells,NK cells and CD4^＋/CD8^＋ratio were significantly higher than the group level before chemotherapy（11.2% ± 0.98% vs 6.7% plus or minus 1.05%,P 0.05; 30% plus or minus 2.01% vs 26%±2.02,P 0.05;10% plus or minus1.02% vs 8.9% plus or minus 0.72%,P〈0.05,1.2±0.03 vs. 0.9±0.06,P〈0.05）,difference before and after chemotherapy and chemotherapy is invalid group were not significant（P〉0.05）.Invalid chemotherapy group B cells in peripheral blood,CD4^＋cells,NK cells and CD4^＋/CD8^＋ratio were significantly lower than normal control group（2.4%±0.01% vs 15% plus or minus 1.10%,P〈0.05;8% plus or minus 0.03% vs37% plus or minus 2.10%,P〈0.05;4.4% plus or minus 0.12%vs 25% plus or minus1.80%,P〈0.05;0.44±0.01vs1.23±0.02,P〈0.05）.Conclusion The NK cells and peripheral blood T lymphocyte subsets for evaluating the effect of colon cancer chemotherapy and prognosis is of important value.