摘要
本研究旨在探讨胰岛素样生长因子1(insulin-like growth factor 1,IGF1)基因SNP与家兔生长性状的关联性。采用DNA池、PCR-SSCP方法对家兔IGF1基因的外显子进行多态性检测,结果发现,在家兔IGF1基因的外显子3中检测到1个SNP位点T365C,表现为3种基因型:TT、TC和CC。IGF1基因不同基因型与家兔生长性状的关联分析结果发现,新西兰兔TT基因型个体的初生重、28日龄体重均显著高于TC和CC基因型个体(P<0.05);比利时兔TT基因型个体的初生重、90日龄体重和初生至90日龄的平均日增重均显著高于TC和CC基因型个体(P<0.05);其余品种的各生长性状指标在该位点均没有达到显著水平(P>0.05)。结果提示,IGF1基因可能是影响家兔生长性状的主效基因或与主效基因连锁,T365C位点有望作为提高家兔个体生长性状的分子遗传标记。
This study was aimed to analyze the association of insulin-like growth factor 1(IGF1)gene polymorphisms with growth traits in rabbit.The technology of DNA pooling,PCR-SSCP and direct sequencing were used to detect the SNP of IGF1 gene.The results showed that T365 C was detected in IGF1 gene exon 3 of rabbit.The SNP(C/T)was devised into three genotypes:TT,TC and CC.The relationships between genotypes and growth traits revealed that the birth weight and the weight at 28 days of age in New Zealand rabbit of TT genotype of T365 Clocus were significantly higher than TC and CC genotypes(P〈0.05),the birth weight,the weight at 90 days of age and average daily gain form birth to 90 days of age in Belgian rabbit of TT genotype of T365 Clocus were significantly higher than TC and CC genotypes(P〈0.05),there were no significant difference of the growth traits in other rabbit breeds(P〈0.05).The consequence indicated that IGF1 gene was primarily deduced to be a potential major gene or linked to major gene effecting the growth traits of rabbit,and this SNP(T365C)might be a candidate molecular genetic markers to improve the growth traits of rabbit.
出处
《中国畜牧兽医》
CAS
北大核心
2017年第8期2378-2385,共8页
China Animal Husbandry & Veterinary Medicine
基金
贵州省科技计划项目(黔科合支撑[2016]2512号)