绵羊卵巢动脉铸型标本制作方法的建立

Making Procedure of Vascular Corrosion Casts of Ovarian Artery in Sheep

试验旨在探讨绵羊卵巢动脉铸型标本的制作方法,研究卵巢及其周边血管的分布与供血特征,为哺乳动物生殖系统血管的精细解剖学尤其是女性卵巢的供血机制研究奠定基础。通过采集绵羊子宫及附件样本,解剖分离出卵巢的部分螺旋动脉使其呈直线型,采用双侧卵巢动脉同时插管,用8%ABS灌注螺旋动脉及其分支,待铸型剂硬化后再用30%盐酸腐蚀软组织,经冲洗、修整获得完整的卵巢动脉血管立体铸型标本。由标本可见,绵羊卵巢动脉分支包括:卵巢动脉卵巢支、卵巢动脉子宫支和卵巢动脉输卵管支;卵巢动脉卵巢支包含:卵巢微动脉、卵巢门螺旋动脉、卵巢门轻螺旋动脉及卵巢门重螺旋动脉。观察发现,卵巢动脉卵巢支呈高度螺旋状结构,卵巢内微动脉主要集中于卵巢门附近,呈高度盘曲折叠的立体网状结构,在微动脉末端发出微血管延伸至卵巢其他区域。通过该方法制成的铸型标本血管走向清晰、表面光滑、立体感强,可作为哺乳动物卵巢血管相关研究的参考。

In order to explore the making procedure of vascular corrosion casts of ovarian artery in sheep and study the distribution of the blood vessels,this study was carried out and it would provide the foundation of blood vessels anatomy of reproductive system including blood supply mechanism of female ovaries in mammals.Firstly,samples of the uterus and appendages in sheep were collected,and then ovarian arteries and their branches were infused with 8% ABS perfusion agents.After the agent became solid,the samples＇ soft tissues were corroded away with 30%hydrochloric acid,washed them down with running water and the moderate trim process was done for obtaining the complete vascular corrosion casts of ovarian artery in sheep.The finished casts showed that the ovarian artery in sheep arises three branches including uterine,ovarian and tubal.The ovarian branch showed complicated arrangement such as the spiral artery of ovarian hilus,light spiral piece of ovarian hilus and tight spiral piece of ovarian branch.The results confirmed that the ovarian branch was characterized by the presence of a tight spiral configuration in sheep,the ovarian arterioles were fasten on ovarian hilus,and there were some dense capillaries which extend to other regions of the ovary followed them.Through this procedure,the vascular distribution of corrosion casts was clear,three-dimensional and with smooth surface.It could provide a reference or model for the study of ovarian blood vessels in mammals.