低糖基化E-钙黏蛋白对Tca8113舌鳞癌细胞增殖的影响

Effects of Low Glycosylated E-cadherin on Proliferation of Tca8113 Squamous Cell Carcinoma of Tongue

目的研究低糖基化的E-钙黏蛋白(E-cadherin)对Tca8113舌鳞癌细胞增殖的影响。方法以Tca8113细胞系作为研究样本,应用含低糖基化的E-cadherin(V13)基因的质粒和含野生型E-cadherin基因的质粒分别转染Tca8113细胞,用western blot实验检测转染基因的稳定表达。通过MTT法和流式细胞术判定V13基因的质粒转染Tca8113细胞对细胞增殖活性的影响;采用SPSS17.0软件包对数据进行统计学分析。结果 (1)Western blot结果显示:空白对照组无标记蛋白表达,V13基因质粒和野生型E-cadherin基因质粒转染Tca8113细胞后均稳定表达,V13的相对分子质量为120 k D小于野生型E-cadherin的相对分子质量150 k D。(2)MTT结果显示:与空白对照组细胞相比,V13基因转染组细胞的增殖活性有明显降低,而野生型E-cadherin转染组细胞的增殖活性无明显改变。(3)流式细胞术结果显示:与空白对照组细胞相比,V13基因转染组细胞的S期比率下调约18.42%,降低明显,而野生型E-cadherin转染组细胞的S期细胞比率改变不明显。结论 V13基因转染比野生型E-cadherin基因转染Tca8113细胞能更显著地抑制该细胞的体外增殖。

Objective The effects of hypoglycosylated E-cadherin gerte on the tongue aquamous cell＇s proliferation after transfected into Tea8113 cells. Method Tca8113 tongue squamous cell lines is our sample. V13 gene and wild-type E-cadherin protein gene were transfected into Tea8113 cell. We use western blot to detect the stable expression of the transfected gene. MTT assay and FACS analysis measured the proliferation of different groups of cells. Result ①Westem blotting： The V13 gene and wild-type E-cadherin gene were stably expressed after transfected. The result showed that molecular weight of V13 was smaller than wild-type E-cadhefin. ②MTT assay： The V13 gene transfected group had a significantly proliferation decrease, while the wild-type E-cadherin gene transfected group had no obvious proliferation change. ③FACS results： The S-phase ratio of V 13 gene transfected cells reduced 18.42%, while no significant change in the wild-type E-cadherin transfected group. Conclusion V13 gene inhibits cell proliferation of Tea8113 cell more obviously than the wild-type E-cadherin gene.