大豆矮化突变体苗期表型观察及对外源激素的响应

Phynotypic Observation and Response to Exogenous Hormones in a Soybean Dwarf-mutant at Seedlings Stage

以‘菏豆12'及其^(60)CO-γ射线诱导矮化突变体为材料,研究突变体的第一复叶叶柄长度、苗期株高、叶柄显微结构,利用4种外源激素处理根系,初步探查突变基因的类型。结果表明:该突变体叶柄比野生型短,株高矮,整体紧凑。叶柄横切观察发现,该突变体木质部细胞及髓细胞直径变小。根系对6-苄氨基嘌呤(6-BA)、赤霉素(GA_3)、吲哚乙酸(IAA)非常敏感,对油菜素内酯(BR)无应答,表明该突变体可能是BR信号转导出现障碍,后续目标基因发掘应围绕BR信号转导途径的相关基因展开。矮化短叶柄突变体在大豆理想株型改造与大豆分子育种中可能具有潜在利用价值。

We adopted the mutant soybean which was generated by the ^60CO-γ ray induce ＇Hedou12＇.Comparative study on the first compound leaf petiole length,seedling plant height and petiole microstructure between wild type and mutant type.In order to explore preliminarily the type of mutation gene,four different hormone treatments were carried out on roots.Result shows that the mutant type has shorter petiole length and lower plant height compared with the wild type,as well as,present a compact phenotype.Petiole crosscutting observation reveal that the mutant cells of xylem and pith become smaller.The mutant roots were very sensitive to 6-BA,GA3,IAA,However,had no response to BR,indicating that the mutant might be BR signal transduction disorder,subsequent target gene discovery should be based on the related genes of BR signal transduction pathway.Soybean dwarf short petiole mutant may have potential application value in the soybean ideotype transformation and soybean molecular breeding.