战骨提取物中柚皮素透皮吸收促进剂的筛选

Screening of transdermal enhancer of naringenin in Premna fulva extract

目的筛选战骨提取物中柚皮素的最佳透皮促渗剂及其用量。方法以大鼠腹部皮肤为实验屏障,用改良Franz扩散池法建立离体经皮吸收实验方法,收集战骨提取物中柚皮素的透皮接收液,用高效液相色谱法测定其透皮速率。结果以冰片、薄荷脑、氮酮作为促渗剂对柚皮素的透皮吸收有不同程度的促进作用,其促渗作用强度冰片>薄荷脑>氮酮,当冰片用量为5%时,柚皮素的透皮速率可达0.136 8 mg/(cm2·h)。结论以5%冰片作为促渗剂时可以较好地促进战骨提取物中柚皮素的经皮吸收。

Objective To screen the best transdermal enhancers of naringenin in Premna fulva extract. Methods Using abdominal skin of rats as experimental barrier, the in vitro percutaneous absorption experiment was established by modified Franz diffusion cell method. The cumulative infiltration and permeation rate of naringenin in three different penetration enhancers were determined by HPLC. Results The borneol, menthol, and azone could enhance transdermal absorption of naringenin in different degree. Several penetration enhancers were increased in the following order： borneol menthol azone. The average percutaneous rate of naringenin was 0.136 8 mg/（cm^2·h）, using 5% borneol as penetration enhancer. Conclusion The 5% borneol is an effective transdermal enhancer for naringenin in P. fulva extract.