三叶青内生真菌Phomopsis sp.YK-7的化学成分研究

Chemical constituents of endophytic fungus Phomopsis sp. YK-7 isolated from Tetrastigma hemsleyanum

目的研究三叶青Tetrastigma hemsleyanum内生真菌Phomopsis sp.YK-7发酵产物中的化学成分。方法采用色谱分离技术进行分离和纯化,并根据谱学数据鉴定化合物的结构。结果从Phomopsis sp.YK-7的发酵产物中分离得到15个化合物,包括13个芳基苯并呋喃类化合物和2个甾体类化合物,分别鉴定为wittifuran X(1)、4-hydroxy-2-(4′-hydroxy-2′-methoxyphenyl)-6-methoxybenzofuran-3-carbaldehyde(2)、erypoegin J(3)、桑辛素S(4)、桑辛素T(5)、桑辛素C(6)、wiffifuran E(7)、iteafuranal A(8)、burttinol D(9)、7-methoxy-2-(4-methoxyphenyl)-3-methyl-5-(3-prenyl)-benzofuran(10)、桑辛素N(11)、桑辛素P(12)、桑辛素M(13)、过氧麦角甾醇(14)和25-hydroxy-ergosta-4,6,8(14),22-tetraen-3-one(15)。结论化合物1~9为首次从该属真菌中分离得到。

Objective To study the chemical constituents of endophytic fungus Phomopsis sp. YK-7 isolated from Tetrastigma hemsleyanum. Methods The compounds were isolated and purified by chromatographic techniques and their structures were identified on the basis of spectral features. Results Fifteent known compounds, including 13 arylbenzofuran derivatives and two steroids, named wittifuran X（1）, 4-hydroxy-2-（4′-hydroxy-2′-methoxyphenyl）-6-methoxybenzofuran-3-carbaldehyde（2）, erypoegin J（3）, moracin S（4）, moracin T（5）, moracin C（6）, wiffifuran E（7）, iteafuranal A（8）, burttinol D（9）, 7-methoxy-2-（4-methoxyphenyl）-3-methyl-5-（3-prenyl）-benzofuran（10）, moracin N（11）, moracin P（12）, moracin M（13）, ergosterol peroxide（14）, and 25-hydroxyergosta-4,6,8（14）,22-tetraen-3-one（15） were isolated from the fermentation products of Phomopsis sp. YK-7. ConclusionCompounds 1—9 are obtained from genus Phomopsis for the first time.