长白山珍稀药材山兰醋酸乙酯部位化学成分研究

Chemical constituents in EtOAc extract from rare medicinal herbs Oreorchas patens in Changbai Mountain

目的研究长白山珍稀药材山兰Oreorchas patens假鳞茎醋酸乙酯部位的化学成分。方法采用硅胶柱色谱、薄层色谱、重结晶、Sephadex LH-20凝胶柱等方法进行分离纯化,通过理化性质及多种波谱数据(~1H-NMR、^(13)C-NMR)鉴定化合物的结构。结果从山兰假鳞茎醋酸乙酯部位分离出11个化合物,分别鉴定为β-谷甾醇(1)、β-胡萝卜苷(2)、邻苯二甲酸二(2-乙基己)酯(3)、邻苯二甲酸-1-丁酯-2-异丁酯(4)、邻苯二甲酸二戊酯(5)、β-谷甾醇亚油酸酯(6)、邻苯二甲酰正二丁酯(7)、对苯二甲酸二丁酯(8)、卷瓣兰蒽(9)、二十五烷(10)、三十六烷(11)。结论化合物3~11均为首次从该属植物中分离得到。

Objective To study the chemical constituents in the rare medicinal herb Oreorchas patens in Changbai Mountain for better utilization of the resource. Methods The drug was used as crude material, the extract was obtained with 95% ethanol firstly, and then with ethyl acetate（Et OAc）. The Et OAc extract was further isolated and purified with various chromatographic methods, including silica gel, ODS, and Sephadex LH-20 columns. Finally the isolated compounds were characterized with their physical chemical properties and based on their ~1H-NMR and ^（13）C-NMR data. Results Eleven compounds were obtained from the Et OAc extract and identified as β-sitosterol（1）, β-daucosterol（2）, dioctyl phthalate（3）, butyl-isobutyl-phthalate（4）, diamyl phthalate（5）, β-sitosterol acetate（6）, dibutyl phthalate（7）, dibutyl terephthalate（8）, cirrhopetalanthin（9）, pentacosane（10）, and hexatriacontane（11）. Conclusion Compounds 3—11 are isolated from O. patens for the first time.