二氢丹参酮抑制人胃癌SGC7901细胞侵袭迁移作用及机制研究

Inhibition of dihydrotanshinone on migration and invasion of gastric cancer SGC7901 cells and its mechanism

目的研究二氢丹参酮(DHT)对人胃癌SGC7901细胞增殖、迁移和侵袭能力的影响,并探讨其作用的分子机制。方法利用不同浓度的DHT处理细胞后,MTT法检测DHT对细胞生长活力的影响,划痕实验观察DHT对细胞运动能力的影响,Transwell小室模型研究DHT对细胞迁移和侵袭的影响,q RT-PCR和Western blotting检测基质金属蛋白酶MMP2、MMP9和Hedgehog通路调控基因Gli1和HHIP的m RNA和蛋白表达水平。结果与对照组相比,DHT可显著抑制SGC7901细胞的体外增殖及迁移能力,Transwell实验表明药物处理后穿膜细胞数明显低于对照组,并且呈剂量依赖性。Western blotting和q RT-PCR实验结果表明,DHT可显著抑制SGC7901细胞中MMP9的表达,降低Gli1基因的m RNA和蛋白表达,并提高HHIP基因的表达水平。结论 DHT能够抑制SGC7901细胞的迁移和侵袭能力,其机制可能与MMP9蛋白的表达降低和Hedgehog通路活性抑制有关。

Objective To study the effects of dihydrotanshinone（DHT） on the migration and invasion of human gastric cancer SGC7901 cells and to investigate its mechanism. Methods SGC7901 cells were treated with different concentration of DHT. Then, the inhibitory effect of DHT was detected by MTT assay. The scratch adhesion test and Transwell assay were performed to determine the migration and invasion capacity of the cells. Quantitative PCR and Western boltting were used to examine the expression of MMP2, MMP9, Gli1, and HHIP in SGC7901 cells. Results DHT could inhibit the proliferation of SGC7901 cells with obvious dose-and time-dependent effects. DHT significantly inhibited the migration and invasion ability in SGC7901 cells in vitro. The expression of MMP9 was obviously down-regulated after DHT treatment. Furthermore, DHT significantly inhibited the Gli1 m RNA and proteins expression levels, and evaluated the expression of HHIP in SGC7901 cells. Conclusion DHT could inhibit the capability of migration and invasion of human gastric cancer SGC7901 cells. The potential molecular mechanism may be related to the inhibition of MMP9, and down-regulation of Hedgehog signaling pathway.