不同发育时期金银花颜色与活性成分的相关性分析

Research on correlation between color and active compounds of Lonicerae Japonicae Flos in different development periods

目的通过探索不同发育时期金银花颜色和每个花蕾活性成分量的相关性,为金银花药材的质量评价提供参考依据。方法采用色度计测量不同发育时期金银花的颜色;HPLC法测定金银花样品中酚酸类、黄酮类、环烯醚萜类等14种化合物的量;采用SPSS 17.0软件分析金银花颜色与化学成分之间的相关性。结果随着花蕾的发育,酚酸类、黄酮类化合物量在二白期、大白期最高,环烯醚萜类化合物量在大白期、银花期最高,从活性成分量结果考虑,金银花的最佳采收期为大白期;金银花发育过程中酚酸类、黄酮类化合物量与亮度(L*)呈显著正相关,酚酸类、黄酮类和环烯醚萜类成分量均与褐变指数(BI)呈极显著负相关。结论金银花的颜色与活性成分量密切相关,L*与BI可以更好地将外观指标与内在质量联系起来。

Objective To provide references for quality evaluation of Lonicerea Japonicae Flos（LJF） through exploring correlation between color and contents of active ingredients of LJF in different development periods. Methods The colorimeter was used to measure the color of LJF in different development periods; HPLC was applied to determine the contents of phenolic acids, flavonoids and iridoids. SPSS 17.0 software was used to analyze the correlation between color and chemical composition of LJF. Results With the development of flower buds, the contents of phenolic acids, flavonoids compounds achieved the highest in two white stages and the contents of iridoids came to the highest in big white stage and silver flower stage. Therefore, only taking the consideration of the content of active ingredient, the best harvest time of LJF might be the big white stage. There was a significant positive correlation among contents of phenolic acids, flavonoids and brightness（L＊） of LJF in different development periods, and there was a very significant negative correlation among contents of phenolic acids, flavonoids, iridoids, and browning index（BI）. Conclusion The color of LJF is closely related to the content of active ingredients, L＊ and BI can better link appearance and inner quality of LJF.