不透明红球菌生产谷氨酸氧化酶发酵过程优化

Optimization of Fermentation Process for Production of Glutamic Acid Oxidase from Rhodococcus opacus

优化了用不透明红球菌FMME1-41所产谷氨酸氧化酶(LGOX)转化L-谷氨酸产α-酮戊二酸(α-KG)的工艺条件.结果表明,最佳发酵培养基为酵母粉6 g/L,大豆蛋白胨2 g/L,(NH_4)_2SO_4 0.8 g/L,葡萄糖25 g/L,KH_2PO_4 3 g/L,MgSO_4 0.6 g/L,MnSO_4 0.3g/L,L-谷氨酸2.5 g/L,在其中发酵30 h,LGOX酶活达6.12 U/mL;在7.5 L发酵罐中优化发酵放大和补料策略,发酵40 h后LGOX酶活达21.5 U/mL;在2 L发酵罐中转化L-谷氨酸生产α-KG,产量达92.0 g/L,摩尔转化率为92.6%,生产强度为9.2 g/(L·h).

The technological conditions for production of a-ketoglutaric acid （tt-KG） from L-glutamic acid were optimized, using L-glutamic oxidase （LGOX） produced by Rhodococcus opacus FMME1-41. The results showed that the optimal nutrition conditions were as follows： 6 g/L yeast powder, 2 g/L soya peptone, 0.8 g/L （NH4）2SO4, 25 g/L glucose, 3 g/L KH2PO4, 0.6 g/L MgSO4, 0.3 g/L MnSO4, 2.5 g/L glutamic acid. The LGOX activity reached 6.12 U/mL after fermentation 30 h. In addition, feeding strategy was optimized in 7.5 L fermentation tank, and the LGOX activity reached 21.5 U/mL after fermentation 40 h. LGOX was used to produce ct-KG from L-glutamic acid in 2 L fermentation tank, the maximum concentration of α-KG reached 92.0 g/L, the molar conversion rate was 92.6% and the production intensity was 9.20 g/（L.h）.