小鼠涎腺发育晚期miRNA基因筛选及功能预测

Screening of Key MiRNAs during Salivary Gland Development in Mice and Functional Prediction.

目的:筛选小鼠涎腺发育晚期差异表达的miRNA,为涎腺组织工程垫定实验基础。方法:应用生物基因芯片构建小鼠涎腺发育胚胎第18天(E18.5)、19天(E19.5)、出生后0d(P0)、出生后3d(P3),4个时期下颌下腺miRNA表达谱,应用GENESPRING10.0进行Quantile normalization。基于Gene Ontology数据库,预测基因的功能。结果:建立小鼠涎腺发育晚期的miRNA表达谱。其中显著变化的有:mmu-miR-133a*,mmu-miR-721,mmu-miR-1,mmu-miR-133b,mmu-miR-133a,mmu-miR-206。结论:显著变化的miRNA在涎腺发育过程中有可能起重要作用。

Objective：To screen differential expression of miRNAs during salivary gland development in mice.Methods：Using microarrays,miRNA expression profiles were established at selected time points during development（E18.5,E19.5,P〈0,P3）of the murine submandibular gland.Furthermore,using GENESPRING10.0,Quantile normalization was established.According to Gene Ontology,the functions were predicted.Results：The expressive profiles of miRNA were established.The miRNAs whose expressive signal strength changed greatly were mmu-miR-133 a,mmu-miR-721,mmu-miR-1,mmu-miR-133 b,mmu-miR-133 a,and mmu-miR-206.Conclusion：The miRNAs whose expressive signal strength changed greatly may play an important role in the salivary gland development.