摘要
目的:筛选小鼠涎腺发育晚期差异表达的miRNA,为涎腺组织工程垫定实验基础。方法:应用生物基因芯片构建小鼠涎腺发育胚胎第18天(E18.5)、19天(E19.5)、出生后0d(P0)、出生后3d(P3),4个时期下颌下腺miRNA表达谱,应用GENESPRING10.0进行Quantile normalization。基于Gene Ontology数据库,预测基因的功能。结果:建立小鼠涎腺发育晚期的miRNA表达谱。其中显著变化的有:mmu-miR-133a*,mmu-miR-721,mmu-miR-1,mmu-miR-133b,mmu-miR-133a,mmu-miR-206。结论:显著变化的miRNA在涎腺发育过程中有可能起重要作用。
Objective:To screen differential expression of miRNAs during salivary gland development in mice.Methods:Using microarrays,miRNA expression profiles were established at selected time points during development(E18.5,E19.5,P〈0,P3)of the murine submandibular gland.Furthermore,using GENESPRING10.0,Quantile normalization was established.According to Gene Ontology,the functions were predicted.Results:The expressive profiles of miRNA were established.The miRNAs whose expressive signal strength changed greatly were mmu-miR-133 a,mmu-miR-721,mmu-miR-1,mmu-miR-133 b,mmu-miR-133 a,and mmu-miR-206.Conclusion:The miRNAs whose expressive signal strength changed greatly may play an important role in the salivary gland development.
出处
《口腔医学研究》
CAS
北大核心
2017年第8期852-855,共4页
Journal of Oral Science Research
关键词
MIRNA
涎腺
基因芯片
MicroRNA Submandibular gland Microarray