红花-甘草配伍对寒凝血瘀大鼠组织中能量代谢的影响

Effect of Carthami Flos-Glycyrrhizae Radix et Rhizoma compatibility on energy metabolism in the tissue of rats with cold coagulation blood stasis

目的:观察红花-甘草配伍对寒凝血瘀模型大鼠组织(心、肝、脾、肺、肾)中磷酸腺苷的代谢,探讨红花-甘草配伍改善寒凝血瘀模型大鼠组织中能量代谢的作用。方法:选用SD大鼠随机分为空白对照组、模型对照组、红花组和红花-甘草组,采用冷刺激的冷水冰浴方法制作寒凝血瘀大鼠模型。于造模成功后开始灌胃给药,每日1次,连续灌服15 d,于给药结束后12 h,取血及心、肝、脾、肺、肾各组织,测定各组大鼠的血液流变学参数,并采用HPLC法测定各组织中三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)的含量。结果:与模型对照组比较,红花组和红花-甘草组的全血粘度、血浆黏度明显降低,ATP、ADP、AMP含量明显增高,且"红花-甘草"组优于红花组(P<0.05)。结论:红花对机体的能量代谢异常有改善作用;甘草能促进红花改善寒凝血瘀疾病的能量代谢障碍。

Objective： To investigate whether the combination of Carthami Flos-Glycyrrhizae Radix et Rhizoma could improve energy metabolism in the tissue of rats with blood stasis syndrome, Carthami Flos-Glycyrrhi- zae Radix et Rhizoma on adenosine phosphate metabolism of tissues including heart, liver, spleen, lung and kidney were observed. Methods： The SD rats were randomly divided into 4 groups, namely blank control group, model group, Carthami Flos group and Carthami Flos-Glycyrrhizae Radix et Rhizoma group. The blood stasis model rats were made with cold stimulation in cold water ice bath. The blank control group and model group were given saline. The Carthami Flos group and Carthami Flos-Glycyrrhizae Radix et Rhizoma group were given aqueous extract of Carthami Flos and aqueous extract of Carthami Flos-Glycyrrhizae Radix et Rhizoma respectively by intragastric administration once per day according to experimental design for 15 days. At the end of the drug administration for 12 h, the blood and tissues （ heart, liver, spleen, lung and kidney ） were obtained and hemorheology parameters of rats were measured. The contents of adenosine triphosphate （ ATP ）, adenosine diphosphate （ ADP ） and adenosine monophosphate （ AMP ） were determined by using high performance liquid chromatography （ HPLC ） method. Results： The whole blood viscosity and plasma viscosity were reduced in the dose group compared with the model group. The contents of adenosine phosphate in model rats after administration of the dose group were higher than those in model group, and the Carthami Flos-Glycyrrhizae Radix et Rhizoma group was better than that of the Carthami Flos group （ P〈0.05 ）. Conclusion： Carthami Flos and Carthami Flos-Glycyrrhizae Radix et Rhizoma herb-pair could improve the energy metabolism in rats with cold coagulation blood stasis, and the effect of Carthami Flos-Glycyrrhizae Radix et Rhizoma was stronger than that of Carthami Flos.